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dc.contributor.authorBeer, KB
dc.contributor.authorFazeli, G
dc.contributor.authorJudasova, K
dc.contributor.authorIrmisch, L
dc.contributor.authorCausemann, J
dc.contributor.authorMansfeld, J
dc.contributor.authorWehman, AM
dc.date.accessioned2020-08-12T11:46:38Z
dc.date.issued2019-08-02
dc.identifier.citationNature communications, 2019, 10 (1), pp. 3490 - ?
dc.identifier.issn2041-1723
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3939
dc.identifier.eissn2041-1723en_US
dc.identifier.doi10.1038/s41467-019-11442-zen_US
dc.description.abstractVisualization of specific organelles in tissues over background fluorescence can be challenging, especially when reporters localize to multiple structures. Instead of trying to identify proteins enriched in specific membrane-wrapped structures, we use a selective degradation approach to remove reporters from the cytoplasm or nucleus of C. elegans embryos and mammalian cells. We demonstrate specific labelling of organelles using degron-tagged reporters, including extracellular vesicles, as well as individual neighbouring membranes. These degron-tagged reporters facilitate long-term tracking of released cell debris and cell corpses, even during uptake and phagolysosomal degradation. We further show that degron protection assays can probe the topology of the nuclear envelope and plasma membrane during cell division, giving insight into protein and organelle dynamics. As endogenous and heterologous degrons are used in bacteria, yeast, plants, and animals, degron approaches can enable the specific labelling and tracking of proteins, vesicles, organelles, cell fragments, and cells in many model systems.
dc.formatElectronic
dc.format.extent3490 - ?
dc.languageeng
dc.language.isoeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHela Cells
dc.subjectCell Membrane
dc.subjectEmbryo, Nonmammalian
dc.subjectAnimals
dc.subjectHumans
dc.subjectCaenorhabditis elegans
dc.subjectLuminescent Proteins
dc.subjectStaining and Labeling
dc.subjectGenes, Reporter
dc.subjectFluorescence
dc.subjectProteolysis
dc.subjectExtracellular Vesicles
dc.subjectIntravital Microscopy
dc.titleDegron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures.
dc.typeJournal Article
dcterms.dateAccepted2019-07-16
rioxxterms.versionofrecord10.1038/s41467-019-11442-z
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2019-08-02en_US
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfNature communications
pubs.issue1
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Post-translational modifications and cell proliferation
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Post-translational modifications and cell proliferation
pubs.publication-statusPublished
pubs.volume10en_US
pubs.embargo.termsNot known
icr.researchteamPost-translational modifications and cell proliferationen_US
dc.contributor.icrauthorMansfeld, Joergen


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