dc.contributor.author | Gheghiani, L | |
dc.contributor.author | Loew, D | |
dc.contributor.author | Lombard, B | |
dc.contributor.author | Mansfeld, J | |
dc.contributor.author | Gavet, O | |
dc.date.accessioned | 2020-08-26T13:26:01Z | |
dc.date.issued | 2017-06-06 | |
dc.identifier.citation | Cell reports, 2017, 19 (10), pp. 2060 - 2073 | |
dc.identifier.issn | 2211-1247 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/4016 | |
dc.identifier.eissn | 2211-1247 | |
dc.identifier.doi | 10.1016/j.celrep.2017.05.031 | |
dc.description.abstract | Commitment to mitosis must be tightly coordinated with DNA replication to preserve genome integrity. While we have previously established that the timely activation of CyclinB1-Cdk1 in late G2 triggers mitotic entry, the upstream regulatory mechanisms remain unclear. Here, we report that Polo-like kinase 1 (Plk1) is required for entry into mitosis during an unperturbed cell cycle and is rapidly activated shortly before CyclinB1-Cdk1. We determine that Plk1 associates with the Cdc25C1 phosphatase and induces its phosphorylation before mitotic entry. Plk1-dependent Cdc25C1 phosphosites are sufficient to promote mitotic entry, even when Plk1 activity is inhibited. Furthermore, we find that activation of Plk1 during G2 relies on CyclinA2-Cdk activity levels. Our findings thus elucidate a critical role for Plk1 in CyclinB1-Cdk1 activation and mitotic entry and outline how CyclinA2-Cdk, an S-promoting factor, poises cells for commitment to mitosis. | |
dc.format | Print | |
dc.format.extent | 2060 - 2073 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | CELL PRESS | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Hela Cells | |
dc.subject | Humans | |
dc.subject | Protein-Serine-Threonine Kinases | |
dc.subject | CDC2 Protein Kinase | |
dc.subject | Cell Cycle Proteins | |
dc.subject | Proto-Oncogene Proteins | |
dc.subject | Mitosis | |
dc.subject | G2 Phase | |
dc.subject | Cyclin B1 | |
dc.subject | Cyclin A2 | |
dc.subject | HEK293 Cells | |
dc.title | PLK1 Activation in Late G2 Sets Up Commitment to Mitosis. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2017-05-09 | |
rioxxterms.versionofrecord | 10.1016/j.celrep.2017.05.031 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2017-06 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Cell reports | |
pubs.issue | 10 | |
pubs.notes | No embargo | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Post-translational modifications and cell proliferation | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Post-translational modifications and cell proliferation | |
pubs.publication-status | Published | |
pubs.volume | 19 | |
pubs.embargo.terms | No embargo | |
icr.researchteam | Post-translational modifications and cell proliferation | |
dc.contributor.icrauthor | Mansfeld, Joerg | |