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dc.contributor.authorZerjatke, T
dc.contributor.authorGak, IA
dc.contributor.authorKirova, D
dc.contributor.authorFuhrmann, M
dc.contributor.authorDaniel, K
dc.contributor.authorGonciarz, M
dc.contributor.authorMüller, D
dc.contributor.authorGlauche, I
dc.contributor.authorMansfeld, J
dc.date.accessioned2020-08-26T13:26:38Z
dc.date.issued2017-05
dc.identifier.citationCell reports, 2017, 19 (9), pp. 1953 - 1966
dc.identifier.issn2211-1247
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4017
dc.identifier.eissn2211-1247
dc.identifier.doi10.1016/j.celrep.2017.05.022
dc.description.abstractCell cycle kinetics are crucial to cell fate decisions. Although live imaging has provided extensive insights into this relationship at the single-cell level, the limited number of fluorescent markers that can be used in a single experiment has hindered efforts to link the dynamics of individual proteins responsible for decision making directly to cell cycle progression. Here, we present fluorescently tagged endogenous proliferating cell nuclear antigen (PCNA) as an all-in-one cell cycle reporter that allows simultaneous analysis of cell cycle progression, including the transition into quiescence, and the dynamics of individual fate determinants. We also provide an image analysis pipeline for automated segmentation, tracking, and classification of all cell cycle phases. Combining the all-in-one reporter with labeled endogenous cyclin D1 and p21 as prime examples of cell-cycle-regulated fate determinants, we show how cell cycle and quantitative protein dynamics can be simultaneously extracted to gain insights into G1 phase regulation and responses to perturbations.
dc.formatPrint
dc.format.extent1953 - 1966
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectDNA Damage
dc.subjectCyclins
dc.subjectProliferating Cell Nuclear Antigen
dc.subjectCell Cycle
dc.subjectG1 Phase
dc.subjectCell Survival
dc.subjectKinetics
dc.subjectGenes, Reporter
dc.subjectCyclin-Dependent Kinase Inhibitor p21
dc.subjectCell Tracking
dc.titleQuantitative Cell Cycle Analysis Based on an Endogenous All-in-One Reporter for Cell Tracking and Classification.
dc.typeJournal Article
dcterms.dateAccepted2017-05-04
rioxxterms.versionofrecord10.1016/j.celrep.2017.05.022
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2017-05
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfCell reports
pubs.issue9
pubs.notesNo embargo
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Post-translational modifications and cell proliferation
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Post-translational modifications and cell proliferation
pubs.publication-statusPublished
pubs.volume19
pubs.embargo.termsNo embargo
icr.researchteamPost-translational modifications and cell proliferationen_US
dc.contributor.icrauthorMansfeld, Joergen


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