dc.contributor.author | George, AL | |
dc.contributor.author | Boulanger, CA | |
dc.contributor.author | Anderson, LH | |
dc.contributor.author | Cagnet, S | |
dc.contributor.author | Brisken, C | |
dc.contributor.author | Smith, GH | |
dc.date.accessioned | 2021-11-30T10:48:22Z | |
dc.date.available | 2021-11-30T10:48:22Z | |
dc.date.issued | 2017-06-15 | |
dc.identifier.citation | Journal of cell science, 2017, 130 (12), pp. 2018 - 2025 | |
dc.identifier.issn | 0021-9533 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/4905 | |
dc.identifier.eissn | 1477-9137 | |
dc.identifier.doi | 10.1242/jcs.200030 | |
dc.description.abstract | Amphiregulin (AREG)-/- mice demonstrate impaired mammary development and form only rudimentary ductal epithelial trees; however, AREG-/- glands are still capable of undergoing alveologenesis and lactogenesis during pregnancy. Transplantation of AREG-/- mammary epithelial cells into cleared mouse mammary fat pads results in a diminished capacity for epithelial growth (∼15%) as compared to that of wild-type mammary epithelial cells. To determine whether estrogen receptor α (ERα, also known as ESR1) and/or AREG signaling were necessary for non-mammary cell redirection, we inoculated either ERα-/- or AREG-/- mammary cells with non-mammary progenitor cells (WAP-Cre/Rosa26LacZ+ male testicular cells or GFP-positive embryonic neuronal stem cells). ERα-/- cells possessed a limited ability to grow or reprogram non-mammary cells in transplanted mammary fat pads. AREG-/- mammary cells were capable of redirecting both types of non-mammary cell populations to mammary phenotypes in regenerating mammary outgrowths. Transplantation of fragments from AREG-reprogrammed chimeric outgrowths resulted in secondary outgrowths in six out of ten fat pads, demonstrating the self-renewing capacity of the redirected non-mammary cells to contribute new progeny to chimeric outgrowths. Nestin was detected at the leading edges of developing alveoli, suggesting that its expression may be essential for lobular expansion. | |
dc.format | Print-Electronic | |
dc.format.extent | 2018 - 2025 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | COMPANY OF BIOLOGISTS LTD | |
dc.rights.uri | https://www.rioxx.net/licenses/all-rights-reserved | |
dc.subject | Testis | |
dc.subject | Spermatozoa | |
dc.subject | Cerebral Cortex | |
dc.subject | Mammary Glands, Animal | |
dc.subject | Epithelial Cells | |
dc.subject | Animals | |
dc.subject | Mice, Transgenic | |
dc.subject | Mice | |
dc.subject | Mice, Nude | |
dc.subject | Green Fluorescent Proteins | |
dc.subject | Estrogen Receptor alpha | |
dc.subject | Estrogens | |
dc.subject | Cell Transplantation | |
dc.subject | Signal Transduction | |
dc.subject | Cell Differentiation | |
dc.subject | Cell Proliferation | |
dc.subject | Cell Lineage | |
dc.subject | Pregnancy | |
dc.subject | Female | |
dc.subject | Male | |
dc.subject | Embryonic Stem Cells | |
dc.subject | Neural Stem Cells | |
dc.subject | Cellular Reprogramming | |
dc.subject | Amphiregulin | |
dc.title | In vivo reprogramming of non-mammary cells to an epithelial cell fate is independent of amphiregulin signaling. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2017-04-24 | |
rioxxterms.version | VoR | |
rioxxterms.versionofrecord | 10.1242/jcs.200030 | |
rioxxterms.licenseref.uri | https://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Journal of cell science | |
pubs.issue | 12 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research/Endocrine control mechanisms | |
pubs.publication-status | Published | |
pubs.volume | 130 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Endocrine control mechanisms | |
dc.contributor.icrauthor | Brisken, Cathrin | |