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dc.contributor.authorPascual-Vargas, P
dc.contributor.authorCooper, S
dc.contributor.authorSero, J
dc.contributor.authorBousgouni, V
dc.contributor.authorArias-Garcia, M
dc.contributor.authorBakal, C
dc.date.accessioned2017-03-24T14:25:36Z
dc.date.issued2017-03
dc.identifier.citationScientific data, 2017, 4 pp. 170018 - ?
dc.identifier.issn2052-4463
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/500
dc.identifier.eissn2052-4463en_US
dc.identifier.doi10.1038/sdata.2017.18en_US
dc.description.abstractIn order to metastasise, triple negative breast cancer (TNBC) must make dynamic changes in cell shape. The shape of all eukaryotic cells is regulated by Rho Guanine Nucleotide Exchange Factors (RhoGEFs), which activate Rho-family GTPases in response to mechanical and informational cues. In contrast, Rho GTPase-activating proteins (RhoGAPs) inhibit Rho GTPases. However, which RhoGEFs and RhoGAPS couple TNBC cell shape to changes in their environment is very poorly understood. Moreover, whether the activity of particular RhoGEFs and RhoGAPs become dysregulated as cells evolve the ability to metastasise is not clear. Towards the ultimate goal of identifying RhoGEFs and RhoGAPs that are essential for TNBC metastasis, we performed an RNAi screen to isolate RhoGEFs and RhoGAPs that contribute to the morphogenesis of the highly metastatic TNBC cell line LM2, and its less-metastatic parental cell line MDA-MB-231. For ~6 million cells from each cell line, we measured 127 different features following the depletion of 142 genes. Using a linear classifier scheme we also describe the morphological heterogeneity of each gene-depleted population.
dc.formatElectronic
dc.format.extent170018 - ?
dc.languageeng
dc.language.isoeng
dc.subjectHumans
dc.subjectGTPase-Activating Proteins
dc.subjectRNA Interference
dc.subjectFemale
dc.subjectTriple Negative Breast Neoplasms
dc.titleRNAi screens for Rho GTPase regulators of cell shape and YAP/TAZ localisation in triple negative breast cancer.
dc.typeJournal Article
dcterms.dateAccepted2016-12-01
rioxxterms.versionofrecord10.1038/sdata.2017.18
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2017-03en_US
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfScientific data
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Dynamical Cell Systems
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Dynamical Cell Systems
pubs.publication-statusPublished
pubs.volume4en_US
pubs.embargo.termsNot known
icr.researchteamDynamical Cell Systemsen_US
dc.contributor.icrauthorBakal, Christopheren
dc.contributor.icrauthorPascual Vargas, Patriciaen


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