Mesenchymal niche remodeling impairs hematopoiesis via stanniocalcin 1 in acute myeloid leukemia.
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Date
2020-06-01ICR Author
Author
Waclawiczek, A
Hamilton, A
Rouault-Pierre, K
Abarrategi, A
Albornoz, MG
Miraki-Moud, F
Bah, N
Gribben, J
Fitzgibbon, J
Taussig, D
Bonnet, D
Type
Journal Article
Metadata
Show full item recordAbstract
Acute myeloid leukemia (AML) disrupts the generation of normal blood cells, predisposing patients to hemorrhage, anemia, and infections. Differentiation and proliferation of residual normal hematopoietic stem and progenitor cells (HSPCs) are impeded in AML-infiltrated bone marrow (BM). The underlying mechanisms and interactions of residual hematopoietic stem cells (HSCs) within the leukemic niche are poorly understood, especially in the human context. To mimic AML infiltration and dissect the cellular crosstalk in human BM, we established humanized ex vivo and in vivo niche models comprising AML cells, normal HSPCs, and mesenchymal stromal cells (MSCs). Both models replicated the suppression of phenotypically defined HSPC differentiation without affecting their viability. As occurs in AML patients, the majority of HSPCs were quiescent and showed enrichment of functional HSCs. HSPC suppression was largely dependent on secreted factors produced by transcriptionally remodeled MSCs. Secretome analysis and functional validation revealed MSC-derived stanniocalcin 1 (STC1) and its transcriptional regulator HIF-1α as limiting factors for HSPC proliferation. Abrogation of either STC1 or HIF-1α alleviated HSPC suppression by AML. This study provides a humanized model to study the crosstalk among HSPCs, leukemia, and their MSC niche, and a molecular mechanism whereby AML impairs normal hematopoiesis by remodeling the mesenchymal niche.
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Subject
Hematopoietic Stem Cells
HL-60 Cells
U937 Cells
Mesenchymal Stem Cells
Animals
Mice, Inbred NOD
Mice, Knockout
Humans
Mice
Mice, SCID
Glycoproteins
Neoplasm Proteins
Hematopoiesis
Female
Male
Hypoxia-Inducible Factor 1, alpha Subunit
Leukemia, Myeloid, Acute
Research team
Acute Leukaemia
Language
eng
Date accepted
2020-02-26
Citation
The Journal of clinical investigation, 2020, 130 (6), pp. 3038 - 3050
Publisher
AMER SOC CLINICAL INVESTIGATION INC