dc.contributor.author | Perea Paizal, J | |
dc.contributor.author | Au, SH | |
dc.contributor.author | Bakal, C | |
dc.coverage.spatial | England | |
dc.date.accessioned | 2024-08-09T11:01:55Z | |
dc.date.available | 2024-08-09T11:01:55Z | |
dc.date.issued | 2024-06-26 | |
dc.identifier | ARTN 14793 | |
dc.identifier | 10.1038/s41598-024-64733-x | |
dc.identifier.citation | Scientific Reports, 2024, 14 (1), pp. 14793 - | en_US |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/6342 | |
dc.identifier.eissn | 2045-2322 | |
dc.identifier.eissn | 2045-2322 | |
dc.identifier.doi | 10.1038/s41598-024-64733-x | |
dc.identifier.doi | 10.1038/s41598-024-64733-x | |
dc.description.abstract | During metastatic dissemination, circulating tumour cells (CTCs) enter capillary beds, where they experience mechanical constriction forces. The transient and persistent effects of these forces on CTCs behaviour remain poorly understood. Here, we developed a high-throughput microfluidic platform mimicking human capillaries to investigate the impact of mechanical constriction forces on malignant and normal breast cell lines. We observed that capillary constrictions induced nuclear envelope rupture in both cancer and normal cells, leading to transient changes in nuclear and cytoplasmic area. Constriction forces transiently activated cGAS/STING and pathways involved in inflammation (NF-κB, STAT and IRF3), especially in the non-malignant cell line. Furthermore, the non-malignant cell line experienced transcriptional changes, particularly downregulation of epithelial markers, while the metastatic cell lines showed minimal alterations. These findings suggest that mechanical constriction forces within capillaries may promote differential effects in malignant and normal cell lines. | |
dc.format | Electronic | |
dc.format.extent | 14793 - | |
dc.language | eng | |
dc.language.iso | eng | en_US |
dc.publisher | NATURE PORTFOLIO | en_US |
dc.relation.ispartof | Scientific Reports | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Humans | |
dc.subject | Breast Neoplasms | |
dc.subject | Female | |
dc.subject | Neoplastic Cells, Circulating | |
dc.subject | Cell Line, Tumor | |
dc.subject | Capillaries | |
dc.subject | Cell Nucleus | |
dc.subject | Neoplasm Metastasis | |
dc.subject | Nuclear Envelope | |
dc.title | Nuclear rupture induced by capillary constriction forces promotes differential effects on metastatic and normal breast cells. | en_US |
dc.type | Journal Article | |
dcterms.dateAccepted | 2024-06-12 | |
dc.date.updated | 2024-08-06T12:57:22Z | |
rioxxterms.version | VoR | en_US |
rioxxterms.versionofrecord | 10.1038/s41598-024-64733-x | en_US |
rioxxterms.licenseref.startdate | 2024-06-26 | |
rioxxterms.type | Journal Article/Review | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/38926422 | |
pubs.issue | 1 | |
pubs.organisational-group | ICR | |
pubs.organisational-group | ICR/Primary Group | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions/Cancer Biology/Dynamical Cell Systems | |
pubs.publication-status | Published online | |
pubs.publisher-url | http://dx.doi.org/10.1038/s41598-024-64733-x | |
pubs.volume | 14 | |
icr.researchteam | Dynamical Cell Systems | en_US |
dc.contributor.icrauthor | Bakal, Christopher | |
icr.provenance | Deposited by Prof Chris Bakal on 2024-08-06. Deposit type is initial. No. of files: 1. Files: Nuclear rupture induced by capillary constriction forces promotes differential effects on metastatic and normal breast cells.pdf | |