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dc.contributor.authorCockle, JVen_US
dc.contributor.authorBrüning-Richardson, Aen_US
dc.contributor.authorScott, KJen_US
dc.contributor.authorThompson, Jen_US
dc.contributor.authorKottke, Ten_US
dc.contributor.authorMorrison, Een_US
dc.contributor.authorIsmail, Aen_US
dc.contributor.authorCarcaboso, AMen_US
dc.contributor.authorRose, Aen_US
dc.contributor.authorSelby, Pen_US
dc.contributor.authorConner, Jen_US
dc.contributor.authorPicton, Sen_US
dc.contributor.authorShort, Sen_US
dc.contributor.authorVile, Ren_US
dc.contributor.authorMelcher, Aen_US
dc.contributor.authorIlett, Een_US
dc.coverage.spatialUnited Statesen_US
dc.date.accessioned2017-10-23T15:13:04Z
dc.date.issued2017-06-16en_US
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/28547002en_US
dc.identifierS2372-7705(17)30019-0en_US
dc.identifier.citationMol Ther Oncolytics, 2017, 5 pp. 75 - 86en_US
dc.identifier.issn2372-7705en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/853
dc.identifier.doi10.1016/j.omto.2017.04.002en_US
dc.description.abstractPediatric high-grade glioma (pHGG) and diffuse intrinsic pontine glioma (DIPG) are invasive tumors with poor survival. Oncolytic virotherapy, initially devised as a direct cytotoxic treatment, is now also known to act via immune-mediated mechanisms. Here we investigate a previously unreported mechanism of action: the inhibition of migration and invasion in pediatric brain tumors. We evaluated the effect of oncolytic herpes simplex virus 1716 (HSV1716) on the migration and invasion of pHGG and DIPG both in vitro using 2D (scratch assay, live cell imaging) and 3D (spheroid invasion in collagen) assays and in vivo using an orthotopic xenograft model of DIPG invasion. HSV1716 inhibited migration and invasion in pHGG and DIPG cell lines. pHGG cells demonstrated reduced velocity and changed morphology in the presence of virus. HSV1716 altered pHGG cytoskeletal dynamics by stabilizing microtubules, inhibiting glycogen synthase kinase-3, and preventing localized clustering of adenomatous polyposis coli (APC) to the leading edge of cells. HSV1716 treatment also reduced tumor infiltration in a mouse orthotopic xenograft DIPG model. Our results demonstrate that HSV1716 targets the migration and invasion of pHGG and DIPG and indicates the potential of an oncolytic virus (OV) to be used as a novel anti-invasive treatment strategy for pediatric brain tumors.en_US
dc.format.extent75 - 86en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectoncolytic virus paediatric brain tumor invasion migrationen_US
dc.titleOncolytic Herpes Simplex Virus Inhibits Pediatric Brain Tumor Migration and Invasion.en_US
dc.typeJournal Article
dcterms.dateAccepted2017-04-25en_US
rioxxterms.versionofrecord10.1016/j.omto.2017.04.002en_US
rioxxterms.licenseref.startdate2017-06-16en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfMol Ther Oncolyticsen_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Translational Immunotherapy
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Translational Immunotherapy/Translational Immunotherapy (TL)
pubs.publication-statusPublished onlineen_US
pubs.volume5en_US
pubs.embargo.termsNot knownen_US
icr.researchteamTranslational Immunotherapyen_US
dc.contributor.icrauthorMelcher, Alanen_US


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/