Now showing items 35-54 of 89

    • Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA. 

      Kong, M; Cutts, EE; Pan, D; Beuron, F; Kaliyappan, T; Xue, C; Morris, EP; Musacchio, A; Vannini, A; Greene, EC (2020-07)
      Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin ...
    • Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection. 

      Ho, JSY; Angel, M; Ma, Y; Sloan, E; Wang, G; Martinez-Romero, C; Alenquer, M; Roudko, V; Chung, L; Zheng, S; Chang, M; Fstkchyan, Y; Clohisey, S; Dinan, AM; Gibbs, J; Gifford, R; Shen, R; Gu, Q; Irigoyen, N; Campisi, L; Huang, C; Zhao, N; Jones, JD; van Knippenberg, I; Zhu, Z; Moshkina, N; Meyer, L; Noel, J; Peralta, Z; Rezelj, V; Kaake, R; Rosenberg, B; Wang, B; Wei, J; Paessler, S; Wise, HM; Johnson, J; Vannini, A; Amorim, MJ; Baillie, JK; Miraldi, ER; Benner, C; Brierley, I; Digard, P; Łuksza, M; Firth, AE; Krogan, N; Greenbaum, BD; MacLeod, MK; van Bakel, H; Garcìa-Sastre, A; Yewdell, JW; Hutchinson, E; Marazzi, I (2020-06-18)
      RNA viruses are a major human health threat. The life cycles of many highly pathogenic RNA viruses like influenza A virus (IAV) and Lassa virus depends on host mRNA, because viral polymerases cleave 5'-m7G-capped host ...
    • Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach. 

      Pollock, K; Ranes, M; Collins, I; Guettler, S (2017-01)
      The poly(ADP-ribose)polymerase (PARP) enzyme tankyrase (TNKS/ARTD5, TNKS2/ARTD6) uses its ankyrin repeat clusters (ARCs) to recognize degenerate peptide motifs in a wide range of proteins, thereby recruiting such proteins ...
    • Induction of detrimental aneuploidy in basal breast cancer cells treated by MPS1 inhibitors in combination with paclitaxel 

      Anderhub, SJ; Mak, GWY; Faisal, A; Walsh, K; Gurden, M; Day, L; Innocenti, P; Woodward, HL; Westwood, IM; Cheung, K-MJ; Naud, S; Hayes, A; Box, G; de Haven Brandon, A; Valenti, MR; O'Fee, L; Saville, H; Burke, R; van Montfort, RLM; Raynaud, FI; Eccles, SA; Blagg, J; Hoelder, S; Linardopoulos, S (AMER ASSOC CANCER RESEARCH, 2017-07-01)
    • Inhibition of mTOR-kinase destabilizes MYCN and is a potential therapy for MYCN-dependent tumors. 

      Vaughan, L; Clarke, PA; Barker, K; Chanthery, Y; Gustafson, CW; Tucker, E; Renshaw, J; Raynaud, F; Li, X; Burke, R; Jamin, Y; Robinson, SP; Pearson, A; Maira, M; Weiss, WA; Workman, P; Chesler, L (2016-09)
      MYC oncoproteins deliver a potent oncogenic stimulus in several human cancers, making them major targets for drug development, but efforts to deliver clinically practical therapeutics have not yet been realized. In childhood ...
    • The Interacting Head Motif Structure Does Not Explain the X-Ray Diffraction Patterns in Relaxed Vertebrate (Bony Fish) Skeletal Muscle and Insect (<i>Lethocerus</i>) Flight Muscle. 

      Knupp, C; Morris, E; Squire, JM (2019-09-14)
      Unlike electron microscopy, which can achieve very high resolution but to date can only be used to study static structures, time-resolved X-ray diffraction from contracting muscles can, in principle, be used to follow the ...
    • An Irreversible Inhibitor of HSP72 that Unexpectedly Targets Lysine-56. 

      Pettinger, J; Le Bihan, Y-V; Widya, M; van Montfort, RLM; Jones, K; Cheeseman, MD (2017-03)
      The stress-inducible molecular chaperone, HSP72, is an important therapeutic target in oncology, but inhibiting this protein with small molecules has proven particularly challenging. Validating HSP72 inhibitors in cells ...
    • Mechanism for remodelling of the cell cycle checkpoint protein MAD2 by the ATPase TRIP13. 

      Alfieri, C; Chang, L; Barford, D (2018-07-04)
      The maintenance of genome stability during mitosis is coordinated by the spindle assembly checkpoint (SAC) through its effector the mitotic checkpoint complex (MCC), an inhibitor of the anaphase-promoting complex (APC/C, ...
    • Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters. 

      Dergai, O; Cousin, P; Gouge, J; Satia, K; Praz, V; Kuhlman, T; Lhôte, P; Vannini, A; Hernandez, N (2018-05-21)
      RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and "proximal sequence element" (PSE), which recruits the ...
    • MIR21 Drives Resistance to Heat Shock Protein 90 Inhibition in Cholangiocarcinoma. 

      Lampis, A; Carotenuto, P; Vlachogiannis, G; Cascione, L; Hedayat, S; Burke, R; Clarke, P; Bosma, E; Simbolo, M; Scarpa, A; Yu, S; Cole, R; Smyth, E; Mateos, JF; Begum, R; Hezelova, B; Eltahir, Z; Wotherspoon, A; Fotiadis, N; Bali, MA; Nepal, C; Khan, K; Stubbs, M; Hahne, JC; Gasparini, P; Guzzardo, V; Croce, CM; Eccles, S; Fassan, M; Cunningham, D; Andersen, JB; Workman, P; Valeri, N; Braconi, C (2018-03)
      BACKGROUND & AIMS:Cholangiocarcinomas (CCA) are resistant to chemotherapy, so new therapeutic agents are needed. We performed a screen to identify small-molecule compounds that are active against CCAs. Levels of microRNA ...
    • MOB1 Mediated Phospho-recognition in the Core Mammalian Hippo Pathway. 

      Couzens, AL; Xiong, S; Knight, JDR; Mao, DY; Guettler, S; Picaud, S; Kurinov, I; Filippakopoulos, P; Sicheri, F; Gingras, A-C (2017-06)
      The Hippo tumor suppressor pathway regulates organ size and tissue homoeostasis in response to diverse signaling inputs. The core of the pathway consists of a short kinase cascade: MST1 and MST2 phosphorylate and activate ...
    • Molecular basis of APC/C regulation by the spindle assembly checkpoint. 

      Alfieri, C; Chang, L; Zhang, Z; Yang, J; Maslen, S; Skehel, M; Barford, D (2016-08-10)
      In the dividing eukaryotic cell, the spindle assembly checkpoint (SAC) ensures that each daughter cell inherits an identical set of chromosomes. The SAC coordinates the correct attachment of sister chromatid kinetochores ...
    • Molecular mechanism of APC/C activation by mitotic phosphorylation. 

      Zhang, S; Chang, L; Alfieri, C; Zhang, Z; Yang, J; Maslen, S; Skehel, M; Barford, D (2016-05)
      In eukaryotes, the anaphase-promoting complex (APC/C, also known as the cyclosome) regulates the ubiquitin-dependent proteolysis of specific cell-cycle proteins to coordinate chromosome segregation in mitosis and entry ...
    • Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation. 

      Gouge, J; Guthertz, N; Kramm, K; Dergai, O; Abascal-Palacios, G; Satia, K; Cousin, P; Hernandez, N; Grohmann, D; Vannini, A (2017-07-25)
      Initiation of gene transcription by RNA polymerase (Pol) III requires the activity of TFIIIB, a complex formed by Brf1 (or Brf2), TBP (TATA-binding protein), and Bdp1. TFIIIB is required for recruitment of Pol III and to ...
    • Molecular mechanisms of human IRE1 activation through dimerization and ligand binding. 

      Joshi, A; Newbatt, Y; McAndrew, PC; Stubbs, M; Burke, R; Richards, MW; Bhatia, C; Caldwell, JJ; McHardy, T; Collins, I; Bayliss, R (2015-05)
      IRE1 transduces the unfolded protein response by splicing XBP1 through its C-terminal cytoplasmic kinase-RNase region. IRE1 autophosphorylation is coupled to RNase activity through formation of a back-to-back dimer, although ...
    • Multiparameter Lead Optimization to Give an Oral Checkpoint Kinase 1 (CHK1) Inhibitor Clinical Candidate: (R)-5-((4-((Morpholin-2-ylmethyl)amino)-5-(trifluoromethyl)pyridin-2-yl)amino)pyrazine-2-carbonitrile (CCT245737). 

      Osborne, JD; Matthews, TP; McHardy, T; Proisy, N; Cheung, K-MJ; Lainchbury, M; Brown, N; Walton, MI; Eve, PD; Boxall, KJ; Hayes, A; Henley, AT; Valenti, MR; De Haven Brandon, AK; Box, G; Jamin, Y; Robinson, SP; Westwood, IM; van Montfort, RLM; Leonard, PM; Lamers, MBAC; Reader, JC; Aherne, GW; Raynaud, FI; Eccles, SA; Garrett, MD; Collins, I (2016-06)
      Multiparameter optimization of a series of 5-((4-aminopyridin-2-yl)amino)pyrazine-2-carbonitriles resulted in the identification of a potent and selective oral CHK1 preclinical development candidate with in vivo efficacy ...
    • Myosin and Actin Filaments in Muscle: Structures and Interactions. 

      Squire, JM; Paul, DM; Morris, EP (2017-01)
      In the last decade, improvements in electron microscopy and image processing have permitted significantly higher resolutions to be achieved (sometimes <1 nm) when studying isolated actin and myosin filaments. In the case ...
    • Nanostructures from Synthetic Genetic Polymers. 

      Taylor, AI; Beuron, F; Peak-Chew, S-Y; Morris, EP; Herdewijn, P; Holliger, P (2016-06)
      Nanoscale objects of increasing complexity can be constructed from DNA or RNA. However, the scope of potential applications could be enhanced by expanding beyond the moderate chemical diversity of natural nucleic acids. ...
    • New tricks for an old dog: Brf2-dependent RNA Polymerase III transcription in oxidative stress and cancer. 

      Gouge, J; Vannini, A (2018-01)
      Here, we discuss the role of Brf2, an RNA Polymerase III core transcription factor, as a master switch of the oxidative stress response. We highlight the interplay of Brf2 with the Nrf2/Keap1 pathway, as well as the role ...
    • Nse5/6 is a negative regulator of the ATPase activity of the Smc5/6 complex. 

      Hallett, ST; Schellenberger, P; Zhou, L; Beuron, F; Morris, E; Murray, JM; Oliver, AW
      The multi-component Smc5/6 complex plays a critical role in the resolution of recombination intermediates formed during mitosis and meiosis, and in the cellular response to replication stress. Using recombinant proteins, ...