A Dynamical Framework for the All-or-None G1/S Transition.
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Embargo End Date
ICR Authors
Authors
Barr, AR
Heldt, FS
Zhang, T
Bakal, C
Novák, B
Heldt, FS
Zhang, T
Bakal, C
Novák, B
Document Type
Journal Article
Date
2016-01-27
Date Accepted
2016-01-04
Abstract
The transition from G1 into DNA replication (S phase) is an emergent behavior resulting from dynamic and complex interactions between cyclin-dependent kinases (Cdks), Cdk inhibitors (CKIs), and the anaphase-promoting complex/cyclosome (APC/C). Understanding the cellular decision to commit to S phase requires a quantitative description of these interactions. We apply quantitative imaging of single human cells to track the expression of G1/S regulators and use these data to parametrize a stochastic mathematical model of the G1/S transition. We show that a rapid, proteolytic, double-negative feedback loop between Cdk2:Cyclin and the Cdk inhibitor p27(Kip1) drives a switch-like entry into S phase. Furthermore, our model predicts that increasing Emi1 levels throughout S phase are critical in maintaining irreversibility of the G1/S transition, which we validate using Emi1 knockdown and live imaging of G1/S reporters. This work provides insight into the general design principles of the signaling networks governing the temporally abrupt transitions between cell-cycle phases.
Citation
Cell systems, 2016, 2 (1), pp. 27 - 37
Source Title
Publisher
CELL PRESS
ISSN
2405-4712
eISSN
2405-4720
Collections
Research Team
Dynamical Cell Systems