Ribosome. The complete structure of the 55S mammalian mitochondrial ribosome.
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Embargo End Date
ICR Authors
Authors
Greber, BJ
Bieri, P
Leibundgut, M
Leitner, A
Aebersold, R
Boehringer, D
Ban, N
Bieri, P
Leibundgut, M
Leitner, A
Aebersold, R
Boehringer, D
Ban, N
Document Type
Journal Article
Date
2015-04-17
Date Accepted
2015-03-06
Abstract
Mammalian mitochondrial ribosomes (mitoribosomes) synthesize mitochondrially encoded membrane proteins that are critical for mitochondrial function. Here we present the complete atomic structure of the porcine 55S mitoribosome at 3.8 angstrom resolution by cryo-electron microscopy and chemical cross-linking/mass spectrometry. The structure of the 28S subunit in the complex was resolved at 3.6 angstrom resolution by focused alignment, which allowed building of a detailed atomic structure including all of its 15 mitoribosomal-specific proteins. The structure reveals the intersubunit contacts in the 55S mitoribosome, the molecular architecture of the mitoribosomal messenger RNA (mRNA) binding channel and its interaction with transfer RNAs, and provides insight into the highly specialized mechanism of mRNA recruitment to the 28S subunit. Furthermore, the structure contributes to a mechanistic understanding of aminoglycoside ototoxicity.
Citation
Science (New York, N.Y.), 2015, 348 (6232), pp. 303 - 308
Source Title
Publisher
AMER ASSOC ADVANCEMENT SCIENCE
ISSN
0036-8075
eISSN
1095-9203