Development of synchronous VHL syndrome tumors reveals contingencies and constraints to tumor evolution.
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ICR Authors
Authors
Fisher, R
Horswell, S
Rowan, A
Salm, MP
de Bruin, EC
Gulati, S
McGranahan, N
Stares, M
Gerlinger, M
Varela, I
Crockford, A
Favero, F
Quidville, V
André, F
Navas, C
Grönroos, E
Nicol, D
Hazell, S
Hrouda, D
O'Brien, T
Matthews, N
Phillimore, B
Begum, S
Rabinowitz, A
Biggs, J
Bates, PA
McDonald, NQ
Stamp, G
Spencer-Dene, B
Hsieh, JJ
Xu, J
Pickering, L
Gore, M
Larkin, J
Swanton, C
Horswell, S
Rowan, A
Salm, MP
de Bruin, EC
Gulati, S
McGranahan, N
Stares, M
Gerlinger, M
Varela, I
Crockford, A
Favero, F
Quidville, V
André, F
Navas, C
Grönroos, E
Nicol, D
Hazell, S
Hrouda, D
O'Brien, T
Matthews, N
Phillimore, B
Begum, S
Rabinowitz, A
Biggs, J
Bates, PA
McDonald, NQ
Stamp, G
Spencer-Dene, B
Hsieh, JJ
Xu, J
Pickering, L
Gore, M
Larkin, J
Swanton, C
Document Type
Journal Article
Date
2014-08-27
Date Accepted
2014-08-08
Abstract
BACKGROUND: Genomic analysis of multi-focal renal cell carcinomas from an individual with a germline VHL mutation offers a unique opportunity to study tumor evolution. RESULTS: We perform whole exome sequencing on four clear cell renal cell carcinomas removed from both kidneys of a patient with a germline VHL mutation. We report that tumors arising in this context are clonally independent and harbour distinct secondary events exemplified by loss of chromosome 3p, despite an identical genetic background and tissue microenvironment. We propose that divergent mutational and copy number anomalies are contingent upon the nature of 3p loss of heterozygosity occurring early in tumorigenesis. However, despite distinct 3p events, genomic, proteomic and immunohistochemical analyses reveal evidence for convergence upon the PI3K-AKT-mTOR signaling pathway. Four germline tumors in this young patient, and in a second, older patient with VHL syndrome demonstrate minimal intra-tumor heterogeneity and mutational burden, and evaluable tumors appear to follow a linear evolutionary route, compared to tumors from patients with sporadic clear cell renal cell carcinoma. CONCLUSIONS: In tumors developing from a germline VHL mutation, the evolutionary principles of contingency and convergence in tumor development are complementary. In this small set of patients with early stage VHL-associated tumors, there is reduced mutation burden and limited evidence of intra-tumor heterogeneity.
Citation
Genome biology, 2014, 15 (8), pp. 433 - ?
Source Title
Publisher
BMC
ISSN
1474-7596
eISSN
1474-760X
Research Team
Melanoma and Kidney Cancer
Experimental Pathology
Translational Oncogenomics
Experimental Pathology
Translational Oncogenomics