Sub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium Gamete Formation.
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ICR Authors
Authors
Invergo, BM
Brochet, M
Yu, L
Choudhary, J
Beltrao, P
Billker, O
Brochet, M
Yu, L
Choudhary, J
Beltrao, P
Billker, O
Document Type
Journal Article
Date
2017-11-14
Date Accepted
2017-10-18
Abstract
The transmission of malaria parasites to mosquitoes relies on the rapid induction of sexual reproduction upon their ingestion into a blood meal. Haploid female and male gametocytes become activated and emerge from their host cells, and the males enter the cell cycle to produce eight microgametes. The synchronized nature of gametogenesis allowed us to investigate phosphorylation signaling during its first minute in Plasmodium berghei via a high-resolution time course of the phosphoproteome. This revealed an unexpectedly broad response, with proteins related to distinct cell cycle events undergoing simultaneous phosphoregulation. We implicate several protein kinases in the process, and we validate our analyses on the plant-like calcium-dependent protein kinase 4 (CDPK4) and a homolog of serine/arginine-rich protein kinases (SRPK1). Mutants in these kinases displayed distinct phosphoproteomic disruptions, consistent with differences in their phenotypes. The results reveal the central role of protein phosphorylation in the atypical cell cycle regulation of a divergent eukaryote.
Citation
Cell reports, 2017, 21 (7), pp. 2017 - 2029
Source Title
Publisher
CELL PRESS
ISSN
2211-1247
eISSN
2211-1247