Proteomics profiling of interactome dynamics by colocalisation analysis (COLA).
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ICR Authors
Authors
Mardakheh, FK
Sailem, HZ
Kümper, S
Tape, CJ
McCully, RR
Paul, A
Anjomani-Virmouni, S
Jørgensen, C
Poulogiannis, G
Marshall, CJ
Bakal, C
Sailem, HZ
Kümper, S
Tape, CJ
McCully, RR
Paul, A
Anjomani-Virmouni, S
Jørgensen, C
Poulogiannis, G
Marshall, CJ
Bakal, C
Document Type
Journal Article
Date
2016-12-20
Date Accepted
2016-11-01
Abstract
Localisation and protein function are intimately linked in eukaryotes, as proteins are localised to specific compartments where they come into proximity of other functionally relevant proteins. Significant co-localisation of two proteins can therefore be indicative of their functional association. We here present COLA, a proteomics based strategy coupled with a bioinformatics framework to detect protein-protein co-localisations on a global scale. COLA reveals functional interactions by matching proteins with significant similarity in their subcellular localisation signatures. The rapid nature of COLA allows mapping of interactome dynamics across different conditions or treatments with high precision.
Citation
Molecular bioSystems, 2016, 13 (1), pp. 92 - 105
Source Title
Publisher
ROYAL SOC CHEMISTRY
ISSN
1742-206X
eISSN
1742-2051
Research Team
Dynamical Cell Systems
Signalling & Cancer Metabolism
Oncogene
Signalling & Cancer Metabolism
Oncogene