Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore.
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ICR Authors
Authors
Bock, LJ
Pagliuca, C
Kobayashi, N
Grove, RA
Oku, Y
Shrestha, K
Alfieri, C
Golfieri, C
Oldani, A
Dal Maschio, M
Bermejo, R
Hazbun, TR
Tanaka, TU
De Wulf, P
Pagliuca, C
Kobayashi, N
Grove, RA
Oku, Y
Shrestha, K
Alfieri, C
Golfieri, C
Oldani, A
Dal Maschio, M
Bermejo, R
Hazbun, TR
Tanaka, TU
De Wulf, P
Document Type
Journal Article
Date
2012-05-06
Date Accepted
2012-04-02
Abstract
Kinetochores attach the replicated chromosomes to the mitotic spindle and orchestrate their transmission to the daughter cells. Kinetochore-spindle binding and chromosome segregation are mediated by the multi-copy KNL1(Spc105), MIS12(Mtw1) and NDC80(Ndc80) complexes that form the so-called KMN network. KMN-spindle attachment is regulated by the Aurora B(Ipl1) and MPS1(Mps1) kinases. It is unclear whether other mechanisms exist that support KMN activity during the cell cycle. Using budding yeast, we show that kinetochore protein Cnn1 localizes to the base of the Ndc80 complex and promotes a functionally competent configuration of the KMN network. Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases.
Citation
Nature cell biology, 2012, 14 (6), pp. 614 - 624
DOI
Source Title
Publisher
NATURE PUBLISHING GROUP
ISSN
1465-7392
eISSN
1476-4679
Collections
Research Team
Molecular mechanisms of cell cycle regulation