High-dimensional functional phenotyping of preclinical human CAR T cells using mass cytometry.

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1-s2.0-S2666166722000545-main.pdf (4.6 MB)

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ICR Authors

Tape, Christopher

Authors

Michelozzi, IM
Sufi, J
Adejumo, TA
Amrolia, PJ
Tape, CJ
Giustacchini, A

Document Type

Journal Article

Date

Date Accepted

2022-03-18

Abstract

Here, we present a comprehensive protocol for the generation and functional characterization of chimeric antigen receptor (CAR) T cells and their products by mass cytometry in a reproducible and scalable manner. We describe the production of CAR T cells from human peripheral blood mononuclear cells. We then detail a three-step staining protocol with metal-labeled antibodies and the subsequent mass cytometry analysis. This protocol allows simultaneous characterization of CAR T cell intracellular signaling, activation, proliferation, cytokine production, and phenotype in a single assay.

Citation

STAR protocols, 2022, 3 (1), pp. 101174 - ?

DOI

10.1016/j.xpro.2022.101174

URI

https://repository.icr.ac.uk/handle/internal/5153

Rights

https://creativecommons.org/licenses/by-nc-nd/4.0/

Source Title

Publisher

ISSN

2666-1667

eISSN

2666-1667

Collections

Closed Research Teams

Research Team

Oncogene

Notes