Metabolic imaging with hyperpolarized [1-13C] pyruvate in patient-derived preclinical mouse models of breast cancer.

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Metabolic imaging with hyperpolarized [1-sup13supC] pyruvate in patient-derived preclinical mouse models of breast cancer.pdf (5.55 MB)

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ICR Authors

Bruna Cabot, Alejandra

Authors

Ros, S
Wright, AJ
Bruna, A
Caldas, C
Brindle, KM

Document Type

Journal Article

Date

2021-09-17

Date Accepted

2021-09-17

Abstract

13C nuclear spin hyperpolarization can increase the sensitivity of detection in an MRI experiment by more than 10,000-fold. 13C magnetic resonance spectroscopic imaging (MRSI) of hyperpolarized 13C label exchange between injected [1-13C]pyruvate and the endogenous tumor lactate pool can be used clinically to assess tumor grade and response to treatment. We describe here an experimental protocol for using this technique in patient-derived and established cell line xenograft models of breast cancer in the mouse. For complete details on the use and execution of this protocol, please refer to Ros et al. (2020).

Citation

STAR protocols, 2021, 2 (3), pp. 100608 - ?

DOI

10.1016/j.xpro.2021.100608

URI

https://repository.icr.ac.uk/handle/internal/4685

Rights

https://creativecommons.org/licenses/by-nc-nd/4.0

Source Title

Publisher

ELSEVIER

ISSN

2666-1667

eISSN

2666-1667

Collections

Cancer Biology

Research Team

Preclinical Modelling of Paediatric Cancer Evolution
Preclinical Modelling of Paediatric Cancer Evolution

Notes