Detection of circulating tumour cell clusters in human glioblastoma.
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Embargo End Date
ICR Authors
Authors
Krol, I
Castro-Giner, F
Maurer, M
Gkountela, S
Szczerba, BM
Scherrer, R
Coleman, N
Carreira, S
Bachmann, F
Anderson, S
Engelhardt, M
Lane, H
Evans, TRJ
Plummer, R
Kristeleit, R
Lopez, J
Aceto, N
Castro-Giner, F
Maurer, M
Gkountela, S
Szczerba, BM
Scherrer, R
Coleman, N
Carreira, S
Bachmann, F
Anderson, S
Engelhardt, M
Lane, H
Evans, TRJ
Plummer, R
Kristeleit, R
Lopez, J
Aceto, N
Document Type
Journal Article
Date
2018-08-14
Date Accepted
2018-06-25
Abstract
Human glioblastoma (GBM) is a highly aggressive, invasive and hypervascularised malignant brain cancer. Individual circulating tumour cells (CTCs) are sporadically found in GBM patients, yet it is unclear whether multicellular CTC clusters are generated in this disease and whether they can bypass the physical hurdle of the blood-brain barrier. Here, we assessed CTC presence and composition at multiple time points in 13 patients with progressing GBM during an open-label phase 1/2a study with the microtubule inhibitor BAL101553. We observe CTC clusters ranging from 2 to 23 cells and present at multiple sampling time points in a GBM patient with pleomorphism and extensive necrosis, throughout disease progression. Exome sequencing of GBM CTC clusters highlights variants in 58 cancer-associated genes including ATM, PMS2, POLE, APC, XPO1, TFRC, JAK2, ERBB4 and ALK. Together, our findings represent the first evidence of the presence of CTC clusters in GBM.
Citation
British journal of cancer, 2018, 119 (4), pp. 487 - 491
Source Title
Publisher
NATURE PUBLISHING GROUP
ISSN
0007-0920
eISSN
1532-1827
Collections
Research Team
Medicine (de Bono Prostate)
Molecular Addictions
Cancer Biomarkers
Molecular Addictions
Cancer Biomarkers