SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay

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ICR Authors

Turajlic, Samra

Authors

Buck, MD
Poirier, EZ
Cardoso, A
Frederico, B
Canton, J
Barrell, S
Beale, R
Byrne, R
Caidan, S
Crawford, M
Cubitt, L
Gandhi, S
Goldstone, R
Grant, PR
Gulati, K
Hindmarsh, S
Howell, M
Hubank, M
Instrell, R
Jiang, M
Kassiotis, G
Lu, W-T
MacRae, JI
Martini, I
Miller, D
Moore, D
Nastouli, E
Nicod, J
Nightingale, L
Olsen, J
Oomatia, A
O'Reilly, N
Rideg, A
Song, O-R
Strange, A
Swanton, C
Turajlic, S
Wu, M
Reis e Sousa, C

Document Type

Journal Article

Date

2021-01-21

Date Accepted

2021-01-21

Abstract

<ns4:p>The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a diagnostic testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning of April 2020. However, due to ongoing demand and competition for critical resources, alternative testing strategies were sought. In this work, we present a clinically-validated procedure for high-throughput SARS-CoV-2 detection by RT-LAMP in 25 minutes that is robust, reliable, repeatable, sensitive, specific, and inexpensive.</ns4:p>

Citation

Wellcome Open Research, 6 pp. 9 - 9

DOI

10.12688/wellcomeopenres.16517.1

URI

https://repository.icr.ac.uk/handle/internal/4513

Rights

https://creativecommons.org/licenses/by/4.0

Source Title

Publisher

F1000 Research Ltd

ISSN

eISSN

2398-502X

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Other ICR Research

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Notes