PLK1 Activation in Late G2 Sets Up Commitment to Mitosis.
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Embargo End Date
ICR Authors
Authors
Gheghiani, L
Loew, D
Lombard, B
Mansfeld, J
Gavet, O
Loew, D
Lombard, B
Mansfeld, J
Gavet, O
Document Type
Journal Article
Date
2017-06-06
Date Accepted
2017-05-09
Abstract
Commitment to mitosis must be tightly coordinated with DNA replication to preserve genome integrity. While we have previously established that the timely activation of CyclinB1-Cdk1 in late G2 triggers mitotic entry, the upstream regulatory mechanisms remain unclear. Here, we report that Polo-like kinase 1 (Plk1) is required for entry into mitosis during an unperturbed cell cycle and is rapidly activated shortly before CyclinB1-Cdk1. We determine that Plk1 associates with the Cdc25C1 phosphatase and induces its phosphorylation before mitotic entry. Plk1-dependent Cdc25C1 phosphosites are sufficient to promote mitotic entry, even when Plk1 activity is inhibited. Furthermore, we find that activation of Plk1 during G2 relies on CyclinA2-Cdk activity levels. Our findings thus elucidate a critical role for Plk1 in CyclinB1-Cdk1 activation and mitotic entry and outline how CyclinA2-Cdk, an S-promoting factor, poises cells for commitment to mitosis.
Citation
Cell reports, 2017, 19 (10), pp. 2060 - 2073
Source Title
Publisher
CELL PRESS
ISSN
2211-1247
eISSN
2211-1247
Collections
Research Team
Post-translational modifications and cell proliferation