Nbeal2 interacts with Dock7, Sec16a, and Vac14.

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Embargo End Date

ICR Authors

Pardo Calvo, Maria Mercedes
 Choudhary, Jyoti

Authors

Mayer, L
Jasztal, M
Pardo, M
Aguera de Haro, S
Collins, J
Bariana, TK
Smethurst, PA
Grassi, L
Petersen, R
Nurden, P
Favier, R
Yu, L
Meacham, S
Astle, WJ
Choudhary, J
Yue, WW
Ouwehand, WH
Guerrero, JA

Document Type

Journal Article

Date

2018-03-01

Date Accepted

2017-11-21

Abstract

Mutations in NBEAL2, the gene encoding the scaffolding protein Nbeal2, are causal of gray platelet syndrome (GPS), a rare recessive bleeding disorder characterized by platelets lacking α-granules and progressive marrow fibrosis. We present here the interactome of Nbeal2 with additional validation by reverse immunoprecipitation of Dock7, Sec16a, and Vac14 as interactors of Nbeal2. We show that GPS-causing mutations in its BEACH domain have profound and possible effects on the interaction with Dock7 and Vac14, respectively. Proximity ligation assays show that these 2 proteins are physically proximal to Nbeal2 in human megakaryocytes. In addition, we demonstrate that Nbeal2 is primarily localized in the cytoplasm and Dock7 on the membrane of or in α-granules. Interestingly, platelets from GPS cases and Nbeal2-/- mice are almost devoid of Dock7, resulting in a profound dysregulation of its signaling pathway, leading to defective actin polymerization, platelet activation, and shape change. This study shows for the first time proteins interacting with Nbeal2 and points to the dysregulation of the canonical signaling pathway of Dock7 as a possible cause of the aberrant formation of platelets in GPS cases and Nbeal2-deficient mice.

Citation

Blood, 2018, 131 (9), pp. 1000 - 1011

DOI

10.1182/blood-2017-08-800359

URI

https://repository.icr.ac.uk/handle/internal/5661

Rights

http://www.rioxx.net/licenses/all-rights-reserved

Source Title

Blood

Publisher

AMER SOC HEMATOLOGY

ISSN

0006-4971

eISSN

1528-0020
1528-0020

Collections

Cell and Molecular Biology

Research Team

Functional Proteomics
Prote & Metabolomics Fac

Notes