Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis.
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ICR Authors
Authors
Strauss, B
Harrison, A
Coelho, PA
Yata, K
Zernicka-Goetz, M
Pines, J
Harrison, A
Coelho, PA
Yata, K
Zernicka-Goetz, M
Pines, J
Document Type
Journal Article
Date
2018-01-02
Date Accepted
2017-09-21
Abstract
There is remarkable redundancy between the Cyclin-Cdk complexes that comprise the cell cycle machinery. None of the mammalian A-, D-, or E-type cyclins are required in development until implantation, and only Cdk1 is essential for early cell divisions. Cyclin B1 is essential for development, but whether it is required for cell division is contentious. Here, we used a novel imaging approach to analyze Cyclin B1-null embryos from fertilization onward. We show that Cyclin B1-/- embryos arrest in G2 phase after just two divisions. This is the earliest arrest of any Cyclin known and places Cyclin B1 with cdk1 as the essential regulators of the cell cycle. We reintroduced mutant proteins into this genetically null background to determine why Cyclin B1 is constantly exported from the nucleus. We found that Cyclin B1 must be exported from the nucleus for the cell to prevent premature entry to mitosis, and retaining Cyclin B1-Cdk1 at the plasma membrane precludes entry to mitosis.
Citation
The Journal of cell biology, 2018, 217 (1), pp. 179 - 193
Source Title
Publisher
ROCKEFELLER UNIV PRESS
ISSN
0021-9525
eISSN
1540-8140
Collections
Research Team
Cell Division