A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component.

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Authors

Cutts, EE
Taylor, GC
Pardo, M
Yu, L
Wills, JC
Choudhary, JS
Vannini, A
Wood, AJ

Document Type

Journal Article

Date

2021-01-13

Date Accepted

2021-01-13

Abstract

Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have assessed the specificity of a polyclonal antibody (Bethyl A302-276A) that is commonly used as a probe for NCAPH2, the kleisin subunit of condensin II, in mammalian cells. We find that, in addition to its intended target, this antibody cross-reacts with one or more components of the SWI/SNF family of chromatin remodelling complexes in an NCAPH2-independent manner. This cross-reactivity, with an abundant chromatin-associated factor, is likely to affect the interpretation of protein and chromatin immunoprecipitation experiments that make use of this antibody probe.

Citation

Wellcome open research, 2021, 6 pp. 3 - ?

Source Title

Publisher

F1000 Research Ltd

ISSN

2398-502X

eISSN

2398-502X

Research Team

Functional Proteomics Group
Vannini Group
Functional Proteomics Group
Vannini Group

Notes