Systematic analysis of tumour cell-extracellular matrix adhesion identifies independent prognostic factors in breast cancer.
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Embargo End Date
ICR Authors
Authors
Todd, JR
Ryall, KA
Vyse, S
Wong, JP
Natrajan, RC
Yuan, Y
Tan, A-C
Huang, PH
Ryall, KA
Vyse, S
Wong, JP
Natrajan, RC
Yuan, Y
Tan, A-C
Huang, PH
Document Type
Journal Article
Date
2016-08-17
Date Accepted
2016-07-27
Abstract
Tumour cell-extracellular matrix (ECM) interactions are fundamental for discrete steps in breast cancer progression. In particular, cancer cell adhesion to ECM proteins present in the microenvironment is critical for accelerating tumour growth and facilitating metastatic spread. To assess the utility of tumour cell-ECM adhesion as a means for discovering prognostic factors in breast cancer survival, here we perform a systematic phenotypic screen and characterise the adhesion properties of a panel of human HER2 amplified breast cancer cell lines across six ECM proteins commonly deregulated in breast cancer. We determine a gene expression signature that defines a subset of cell lines displaying impaired adhesion to laminin. Cells with impaired laminin adhesion showed an enrichment in genes associated with cell motility and molecular pathways linked to cytokine signalling and inflammation. Evaluation of this gene set in the Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) cohort of 1,964 patients identifies the F12 and STC2 genes as independent prognostic factors for overall survival in breast cancer. Our study demonstrates the potential of in vitro cell adhesion screens as a novel approach for identifying prognostic factors for disease outcome.
Citation
Oncotarget, 2016, 7 (39), pp. 62939 - 62953
Source Title
Publisher
IMPACT JOURNALS LLC
ISSN
1949-2553
eISSN
1949-2553
Research Team
Protein Networks
Functional Genomics
Molecular and Systems Oncology
Functional Genomics
Molecular and Systems Oncology