Assessing HER2 Amplification in Plasma cfDNA.
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Embargo End Date
ICR Authors
Authors
Garcia-Murillas, I
Turner, NC
Turner, NC
Document Type
Journal Article
Date
2018-05-02
Date Accepted
2018-05-02
Abstract
Digital PCR (dPCR) is a highly accurate method to determine DNA concentration. In dPCR, DNA is portioned into many discrete single entities, and these are analyzed individually for the presence or absence of a target molecule of interest. Here we describe how digital PCR can be employed to determine the presence of oncogenic amplification through noninvasive analysis of circulating free DNA (cfDNA), and exemplify this approach by developing a plasma circulating free DNA dPCR assay for HER2 copy number.
Citation
Methods in molecular biology (Clifton, N.J.), 2018, 1768 pp. 161 - 172
Source Title
Publisher
HUMANA PRESS INC
ISSN
1064-3745
eISSN
1940-6029
Collections
Research Team
Molecular Oncology