Capture Hi-C identifies the chromatin interactome of colorectal cancer risk loci.
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Authors
Jäger, R
Migliorini, G
Henrion, M
Kandaswamy, R
Speedy, HE
Heindl, A
Whiffin, N
Carnicer, MJ
Broome, L
Dryden, N
Nagano, T
Schoenfelder, S
Enge, M
Yuan, Y
Taipale, J
Fraser, P
Fletcher, O
Houlston, RS
Migliorini, G
Henrion, M
Kandaswamy, R
Speedy, HE
Heindl, A
Whiffin, N
Carnicer, MJ
Broome, L
Dryden, N
Nagano, T
Schoenfelder, S
Enge, M
Yuan, Y
Taipale, J
Fraser, P
Fletcher, O
Houlston, RS
Document Type
Journal Article
Date
2015-02-19
Date Accepted
2014-12-30
Abstract
Multiple regulatory elements distant from their targets on the linear genome can influence the expression of a single gene through chromatin looping. Chromosome conformation capture implemented in Hi-C allows for genome-wide agnostic characterization of chromatin contacts. However, detection of functional enhancer-promoter interactions is precluded by its effective resolution that is determined by both restriction fragmentation and sensitivity of the experiment. Here we develop a capture Hi-C (cHi-C) approach to allow an agnostic characterization of these physical interactions on a genome-wide scale. Single-nucleotide polymorphisms associated with complex diseases often reside within regulatory elements and exert effects through long-range regulation of gene expression. Applying this cHi-C approach to 14 colorectal cancer risk loci allows us to identify key long-range chromatin interactions in cis and trans involving these loci.
Citation
Nature communications, 2015, 6 pp. 6178 - ?
Source Title
Publisher
NATURE PORTFOLIO
ISSN
2041-1723
eISSN
2041-1723
Research Team
Functional Genetic Epidemiology
Cancer Genomics
Computational Pathology & Integrated Genomics
Gene Function
Molecular & Population Genetics
Cancer Genomics
Computational Pathology & Integrated Genomics
Gene Function
Molecular & Population Genetics