Identifying Genetic Dependencies in Cancer by Analyzing siRNA Screens in Tumor Cell Line Panels.
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Embargo End Date
ICR Authors
Authors
Campbell, J
Ryan, CJ
Lord, CJ
Ryan, CJ
Lord, CJ
Document Type
Journal Article
Date
2018-01-01
Date Accepted
2017-12-19
Date Available
Abstract
Loss-of-function screening using RNA interference or CRISPR approaches can be used to identify genes that specific tumor cell lines depend upon for survival. By integrating the results from screens in multiple cell lines with molecular profiling data, it is possible to associate the dependence upon specific genes with particular molecular features (e.g., the mutation of a cancer driver gene, or transcriptional or proteomic signature). Here, using a panel of kinome-wide siRNA screens in osteosarcoma cell lines as an example, we describe a computational protocol for analyzing loss-of-function screens to identify genetic dependencies associated with particular molecular features. We describe the steps required to process the siRNA screen data, integrate the results with genotypic information to identify genetic dependencies, and finally the integration of protein-protein interaction data to interpret these dependencies.
Citation
Methods in molecular biology (Clifton, N.J.), 2018, 1711 pp. 83 - 99
Source Title
Publisher
HUMANA PRESS INC
ISSN
1064-3745
eISSN
1940-6029
Collections
Research Team
Gene Function