Rapid autopsies to enhance metastatic research: the UPTIDER post-mortem tissue donation program.
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ICR Authors
Authors
Geukens, T
De Schepper, M
Van Den Bogaert, W
Van Baelen, K
Maetens, M
Pabba, A
Mahdami, A
Leduc, S
Isnaldi, E
Nguyen, H-L
Bachir, I
Hajipirloo, M
Zels, G
Van Cauwenberge, J
Borremans, K
Vandecaveye, V
Weynand, B
Vermeulen, P
Leucci, E
Baietti, MF
Sflomos, G
Battista, L
Brisken, C
Derksen, PWB
Koorman, T
Visser, D
Scheele, CLGJ
Thommen, DS
Hatse, S
Fendt, S-M
Vanderheyden, E
Van Brussel, T
Schepers, R
Boeckx, B
Lambrechts, D
Marano, G
Biganzoli, E
Smeets, A
Nevelsteen, I
Punie, K
Neven, P
Wildiers, H
Richard, F
Floris, G
Desmedt, C
De Schepper, M
Van Den Bogaert, W
Van Baelen, K
Maetens, M
Pabba, A
Mahdami, A
Leduc, S
Isnaldi, E
Nguyen, H-L
Bachir, I
Hajipirloo, M
Zels, G
Van Cauwenberge, J
Borremans, K
Vandecaveye, V
Weynand, B
Vermeulen, P
Leucci, E
Baietti, MF
Sflomos, G
Battista, L
Brisken, C
Derksen, PWB
Koorman, T
Visser, D
Scheele, CLGJ
Thommen, DS
Hatse, S
Fendt, S-M
Vanderheyden, E
Van Brussel, T
Schepers, R
Boeckx, B
Lambrechts, D
Marano, G
Biganzoli, E
Smeets, A
Nevelsteen, I
Punie, K
Neven, P
Wildiers, H
Richard, F
Floris, G
Desmedt, C
Document Type
Journal Article
Date
2024-04-24
Date Accepted
2024-04-05
Abstract
Research on metastatic cancer has been hampered by limited sample availability. Here we present the breast cancer post-mortem tissue donation program UPTIDER and show how it enabled sampling of a median of 31 (range: 5-90) metastases and 5-8 liquids per patient from its first 20 patients. In a dedicated experiment, we show the mild impact of increasing time after death on RNA quality, transcriptional profiles and immunohistochemical staining in tumor tissue samples. We show that this impact can be counteracted by organ cooling. We successfully generated ex vivo models from tissue and liquid biopsies from distinct histological subtypes of breast cancer. We anticipate these and future findings of UPTIDER to elucidate mechanisms of disease progression and treatment resistance and to provide tools for the exploration of precision medicine strategies in the metastatic setting.
Citation
npj Breast Cancer, 2024, 10 (1), pp. 31 -
Source Title
npj Breast Cancer
Publisher
NATURE PORTFOLIO
ISSN
2374-4677
eISSN
2374-4677
2374-4677
2374-4677
Collections
Research Team
Endocrine control mechans