Isolation and Comparative Transcriptome Analysis of Human Fetal and iPSC-Derived Cone Photoreceptor Cells.
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Authors
Welby, E
Lakowski, J
Di Foggia, V
Budinger, D
Gonzalez-Cordero, A
Lun, ATL
Epstein, M
Patel, A
Cuevas, E
Kruczek, K
Naeem, A
Minneci, F
Hubank, M
Jones, DT
Marioni, JC
Ali, RR
Sowden, JC
Lakowski, J
Di Foggia, V
Budinger, D
Gonzalez-Cordero, A
Lun, ATL
Epstein, M
Patel, A
Cuevas, E
Kruczek, K
Naeem, A
Minneci, F
Hubank, M
Jones, DT
Marioni, JC
Ali, RR
Sowden, JC
Document Type
Journal Article
Date
2017-12-12
Date Accepted
2017-10-15
Abstract
Loss of cone photoreceptors, crucial for daylight vision, has the greatest impact on sight in retinal degeneration. Transplantation of stem cell-derived L/M-opsin cones, which form 90% of the human cone population, could provide a feasible therapy to restore vision. However, transcriptomic similarities between fetal and stem cell-derived cones remain to be defined, in addition to development of cone cell purification strategies. Here, we report an analysis of the human L/M-opsin cone photoreceptor transcriptome using an AAV2/9.pR2.1:GFP reporter. This led to the identification of a cone-enriched gene signature, which we used to demonstrate similar gene expression between fetal and stem cell-derived cones. We then defined a cluster of differentiation marker combination that, when used for cell sorting, significantly enriches for cone photoreceptors from the fetal retina and stem cell-derived retinal organoids, respectively. These data may facilitate more efficient isolation of human stem cell-derived cones for use in clinical transplantation studies.
Citation
Stem cell reports, 2017, 9 (6), pp. 1898 - 1915
Source Title
Publisher
CELL PRESS
ISSN
2213-6711
eISSN
2213-6711
Collections
Research Team
Translational Genomics