Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome.
Loading...
Embargo End Date
ICR Authors
Authors
Worboys, JD
Sinclair, J
Yuan, Y
Jørgensen, C
Sinclair, J
Yuan, Y
Jørgensen, C
Document Type
Journal Article
Date
2014-10-01
Date Accepted
2014-07-14
Abstract
In targeted proteomics it is critical that peptides are not only proteotypic but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. We show that measuring ratios of proteotypic peptides across biological samples can be used to empirically identify peptides with good quantotypic properties. We applied this technique to identify quantotypic peptides for 21% of the human kinome.
Citation
Nature methods, 2014, 11 (10), pp. 1041 - 1044
Source Title
Publisher
NATURE PUBLISHING GROUP
ISSN
1548-7091
eISSN
1548-7105
Collections
Research Team
Cell Communication
Computational Pathology & Integrated Genomics
Computational Pathology & Integrated Genomics