Identification of a novel genetic locus associated with immune-mediated thrombotic thrombocytopenic purpura.

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ICR Authors

Hubank, Michael

Authors

Stubbs, MJ
Coppo, P
Cheshire, C
Veyradier, A
Dufek, S
Levine, AP
Thomas, M
Patel, V
Connolly, JO
Hubank, M
Benhamou, Y
Galicier, L
Poullin, P
Kleta, R
Gale, DP
Stanescu, H
Scully, MA

Document Type

Journal Article

Date

2022-03-01

Date Accepted

2021-02-18

Abstract

Immune thrombotic thrombocytopenic purpura (iTTP) is an ultra-rare, life-threatening disorder, mediated through severe ADAMTS13 deficiency causing multi-system micro-thrombi formation, and has specific human leukocyte antigen associations. We undertook a large genome-wide association study to investigate additional genetically distinct associations in iTTP. We compared two iTTP patient cohorts with controls, following standardized genome-wide quality control procedures for single-nucleotide polymorphisms and imputed HLA types. Associations were functionally investigated using expression quantitative trait loci (eQTL), and motif binding prediction software. Independent associations consistent with previous findings in iTTP were detected at the HLA locus and in addition a novel association was detected on chromosome 3 (rs9884090, P=5.22x10-10, odds ratio 0.40) in the UK discovery cohort. Meta-analysis, including the French replication cohort, strengthened the associations. The haploblock containing rs9884090 is associated with reduced protein O-glycosyltransferase 1 (POGLUT1) expression (eQTL P<0.05), and functional annotation suggested a potential causative variant (rs71767581). This work implicates POGLUT1 in iTTP pathophysiology and suggests altered post-translational modification of its targets may influence disease susceptibility.

Citation

Haematologica, 2021

DOI

10.3324/haematol.2020.274639

URI

https://repository.icr.ac.uk/handle/internal/4431

Rights

https://creativecommons.org/licenses/by-nc/4.0

Source Title

Publisher

FERRATA STORTI FOUNDATION

ISSN

0390-6078

eISSN

1592-8721

Collections

Cancer Biology

Research Team

Translational Genomics
Translational Genomics

Notes