Technical note on the exploration of COVID-19 in autopsy material.
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Embargo End Date
ICR Authors
Authors
Humphries, MP
Bingham, V
Abdullah Sidi, F
Craig, S
Lara, B
El-Daly, H
O'Doherty, N
Maxwell, P
Lewis, C
McQuaid, S
Lyness, J
James, J
Snead, DRJ
Salto-Tellez, M
Bingham, V
Abdullah Sidi, F
Craig, S
Lara, B
El-Daly, H
O'Doherty, N
Maxwell, P
Lewis, C
McQuaid, S
Lyness, J
James, J
Snead, DRJ
Salto-Tellez, M
Document Type
Journal Article
Date
2023-01-30
Date Accepted
2022-10-17
Abstract
Interrogation of immune response in autopsy material from patients with SARS-CoV-2 is potentially significant. We aim to describe a validated protocol for the exploration of the molecular physiopathology of SARS-CoV-2 pulmonary disease using multiplex immunofluorescence (mIF).The application of validated assays for the detection of SARS-CoV-2 in tissues, originally developed in our laboratory in the context of oncology, was used to map the topography and complexity of the adaptive immune response at protein and mRNA levels.SARS-CoV-2 is detectable in situ by protein or mRNA, with a sensitivity that could be in part related to disease stage. In formalin-fixed, paraffin-embedded pneumonia material, multiplex immunofluorescent panels are robust, reliable and quantifiable and can detect topographic variations in inflammation related to pathological processes.Clinical autopsies have relevance in understanding diseases of unknown/complex pathophysiology. In particular, autopsy materials are suitable for the detection of SARS-CoV-2 and for the topographic description of the complex tissue-based immune response using mIF.
Citation
Journal of Clinical Pathology, 2023, pp. jcp-2022-208525 -
Source Title
Journal of Clinical Pathology
Publisher
BMJ PUBLISHING GROUP
ISSN
0021-9746
eISSN
1472-4146
1472-4146
1472-4146
Collections
Research Team
Integrated Pathology