Structural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome.
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Embargo End Date
ICR Authors
Authors
Faull, SV
Lau, AMC
Martens, C
Ahdash, Z
Hansen, K
Yebenes, H
Schmidt, C
Beuron, F
Cronin, NB
Morris, EP
Politis, A
Lau, AMC
Martens, C
Ahdash, Z
Hansen, K
Yebenes, H
Schmidt, C
Beuron, F
Cronin, NB
Morris, EP
Politis, A
Document Type
Journal Article
Date
2019-08-23
Date Accepted
2019-08-02
Abstract
Cullin-Ring E3 Ligases (CRLs) regulate a multitude of cellular pathways through specific substrate receptors. The COP9 signalosome (CSN) deactivates CRLs by removing NEDD8 from activated Cullins. Here we present structures of the neddylated and deneddylated CSN-CRL2 complexes by combining single-particle cryo-electron microscopy (cryo-EM) with chemical cross-linking mass spectrometry (XL-MS). These structures suggest a conserved mechanism of CSN activation, consisting of conformational clamping of the CRL2 substrate by CSN2/CSN4, release of the catalytic CSN5/CSN6 heterodimer and finally activation of the CSN5 deneddylation machinery. Using hydrogen-deuterium exchange (HDX)-MS we show that CRL2 activates CSN5/CSN6 in a neddylation-independent manner. The presence of NEDD8 is required to activate the CSN5 active site. Overall, by synergising cryo-EM with MS, we identify sensory regions of the CSN that mediate its stepwise activation and provide a framework for understanding the regulatory mechanism of other Cullin family members.
Citation
Nature communications, 2019, 10 (1), pp. 3814 - ?
Source Title
Publisher
NATURE RESEARCH
ISSN
2041-1723
eISSN
2041-1723
Collections
Research Team
Structural Electron Microscopy