Resveratrol inhibits benzo[a]pyrene–DNA adduct formation in human bronchial epithelial cells
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Embargo End Date
ICR Authors
Authors
Berge, G
Øvrebø, S
Botnen, IV
Hewer, A
Phillips, DH
Haugen, A
Mollerup, S
Øvrebø, S
Botnen, IV
Hewer, A
Phillips, DH
Haugen, A
Mollerup, S
Document Type
Journal Article
Date
2004-07-19
Date Accepted
Abstract
Resveratrol ( trans-3,4’,5-trihydroxystilbene), a phytoalexin present in various plants and foods, has in several in vitro and in vivo studies demonstrated cancer chemopreventive and chemotherapeutic potential. We investigated the in vitro effect of resveratrol on benzo[ a] pyrene ( B[ a] P)-induced DNA adducts in human bronchial epithelial cells. This was compared to the effect of resveratrol on the expression of the cytochrome P450 (CYP) genes CYP1A1 and CYP1B1 and the formation of B[ a] P metabolites. Exposure of BEAS-2B and BEP2D cells to B[ a] P and increasing concentrations of resveratrol resulted in a dose- and time-dependent inhibition of DNA adduct formation quantified by P-32-postlabelling. Supporting this result, resveratrol was shown to inhibit CYP1A1 and CYP1B1 gene expression, as measured by real-time reverse transcriptase - polymerase chain reaction. Also, a significant correlation was found between the number of DNA adducts and the mRNA levels of these genes. Using HPLC analysis, a concomitant decrease in the formation of B[ a]P-derived metabolic products was detected. In conclusion, these data lend support to a chemopreventive role of resveratrol in polycyclic aromatic hydrocarbon-induced carcinogenesis.
Citation
BRITISH JOURNAL OF CANCER, 2004, 91 pp. 333 - 338
Source Title
Publisher
Springer Science and Business Media LLC
ISSN
0007-0920
eISSN
Collections
Research Team
Human Biomonitoring & Carcinogen Activation