An integrated model for termination of RNA polymerase III transcription.
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ICR Authors
Authors
Xie, J
Aiello, U
Clement, Y
Haidara, N
Girbig, M
Schmitzova, J
Pena, V
Müller, CW
Libri, D
Porrua, O
Aiello, U
Clement, Y
Haidara, N
Girbig, M
Schmitzova, J
Pena, V
Müller, CW
Libri, D
Porrua, O
Document Type
Journal Article
Date
2022-07-15
Date Accepted
2022-05-26
Abstract
RNA polymerase III (RNAPIII) synthesizes essential and abundant noncoding RNAs such as transfer RNAs. Controlling RNAPIII span of activity by accurate and efficient termination is a challenging necessity to ensure robust gene expression and to prevent conflicts with other DNA-associated machineries. The mechanism of RNAPIII termination is believed to be simpler than that of other eukaryotic RNA polymerases, solely relying on the recognition of a T-tract in the nontemplate strand. Here, we combine high-resolution genome-wide analyses and in vitro transcription termination assays to revisit the mechanism of RNAPIII transcription termination in budding yeast. We show that T-tracts are necessary but not always sufficient for termination and that secondary structures of the nascent RNAs are important auxiliary cis-acting elements. Moreover, we show that the helicase Sen1 plays a key role in a fail-safe termination pathway. Our results provide a comprehensive model illustrating how multiple mechanisms cooperate to ensure efficient RNAPIII transcription termination.
Citation
Science Advances, 2022, 8 (28), pp. eabm9875 -
Source Title
Science Advances
Publisher
American Association for the Advancement of Science (AAAS)
ISSN
2375-2548
eISSN
2375-2548
2375-2548
2375-2548
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Research Team
Mech of pre-mRNA splicing