Toward a real liquid biopsy in metastatic breast and prostate cancer: Diagnostic LeukApheresis increases CTC yields in a European prospective multicenter study (CTCTrap).
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ICR Authors
Authors
Andree, KC
Mentink, A
Zeune, LL
Terstappen, LWMM
Stoecklein, NH
Neves, RP
Driemel, C
Lampignano, R
Yang, L
Neubauer, H
Fehm, T
Fischer, JC
Rossi, E
Manicone, M
Basso, U
Marson, P
Zamarchi, R
Loriot, Y
Lapierre, V
Faugeroux, V
Oulhen, M
Farace, F
Fowler, G
Sousa Fontes, M
Ebbs, B
Lambros, M
Crespo, M
Flohr, P
de Bono, JS
Mentink, A
Zeune, LL
Terstappen, LWMM
Stoecklein, NH
Neves, RP
Driemel, C
Lampignano, R
Yang, L
Neubauer, H
Fehm, T
Fischer, JC
Rossi, E
Manicone, M
Basso, U
Marson, P
Zamarchi, R
Loriot, Y
Lapierre, V
Faugeroux, V
Oulhen, M
Farace, F
Fowler, G
Sousa Fontes, M
Ebbs, B
Lambros, M
Crespo, M
Flohr, P
de Bono, JS
Document Type
Journal Article
Date
2018-11-15
Date Accepted
2018-06-25
Abstract
Frequently, the number of circulating tumor cells (CTC) isolated in 7.5 mL of blood is too small to reliably determine tumor heterogeneity and to be representative as a "liquid biopsy". In the EU FP7 program CTCTrap, we aimed to validate and optimize the recently introduced Diagnostic LeukApheresis (DLA) to screen liters of blood. Here we present the results obtained from 34 metastatic cancer patients subjected to DLA in the participating institutions. About 7.5 mL blood processed with CellSearch® was used as "gold standard" reference. DLAs were obtained from 22 metastatic prostate and 12 metastatic breast cancer patients at four different institutions without any noticeable side effects. DLA samples were prepared and processed with different analysis techniques. Processing DLA using CellSearch resulted in a 0-32 fold increase in CTC yield compared to processing 7.5 mL blood. Filtration of DLA through 5 μm pores microsieves was accompanied by large CTC losses. Leukocyte depletion of 18 mL followed by CellSearch yielded an increase of the number of CTC but a relative decrease in yield (37%) versus CellSearch DLA. In four out of seven patients with 0 CTC detected in 7.5 mL of blood, CTC were detected in DLA (range 1-4 CTC). The CTC obtained through DLA enables molecular characterization of the tumor. CTC enrichment technologies however still need to be improved to isolate all the CTC present in the DLA.
Citation
International journal of cancer, 2018, 143 (10), pp. 2584 - 2591
Source Title
Publisher
WILEY
ISSN
0020-7136
eISSN
1097-0215
Collections
Research Team
Cancer Biomarkers
Prostate Cancer Targeted Therapy Group
Prostate Cancer Targeted Therapy Group