Magnetic Resonance Spectroscopy to Study Glycolytic Metabolism During Autophagy.
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Embargo End Date
ICR Authors
Authors
Chung, Y-L
Leach, MO
Eykyn, TR
Leach, MO
Eykyn, TR
Document Type
Journal Article
Date
2017-01-01
Date Accepted
Abstract
Cancer cells undergoing starvation- and treatment-induced autophagy were found to exhibit reduced intracellular lactate, reduced rates of steady-state lactate excretion and reduced real-time pyruvate-lactate exchange rates, indicating that glycolytic metabolism was altered in autophagic cells. In this chapter, we describe the technical details of the use of 1H-magnetic resonance spectroscopy (MRS) to measure endogenous cellular concentrations of lactate and glucose in autophagic cells and tissues, how to measure the rate of steady-state lactate excretion and glucose uptake by 1H-MRS in autophagic cells, and details of the real-time measurement of [1-13C] pyruvate to lactate exchange in autophagic cells by 13C-MRS-DNP (dynamic nuclear polarization).
Citation
Methods in enzymology, 2017, 588 pp. 133 - 153
Source Title
Publisher
ELSEVIER ACADEMIC PRESS INC
ISSN
0076-6879
eISSN
1557-7988
Collections
Research Team
Magnetic Resonance