Dual-view oblique plane microscopy (dOPM).
Embargo End Date
ICR Authors
Authors
Sparks, H
Dent, L
Bakal, C
Behrens, A
Salbreux, G
Dunsby, C
Dent, L
Bakal, C
Behrens, A
Salbreux, G
Dunsby, C
Document Type
Journal Article
Date
2020-12-01
Date Accepted
2020-10-28
Abstract
We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a water immersion 40× 1.15 NA primary objective, deconvolved image volumes of 200 nm beads were measured to have full width at half maxima (FWHM) of 0.35 ± 0.04 µm and 0.39 ± 0.02 µm laterally and 0.81 ± 0.07 µm axially. The measured z-sectioning value was 1.33 ± 0.45 µm using light-sheet FWHM in the frames of the two views of 4.99 ± 0.58 µm and 4.89 ± 0.63 µm. To qualitatively demonstrate that the system can reduce shadow artefacts while providing a more isotropic resolution, a multi-cellular spheroid approximately 100 µm in diameter was imaged.
Citation
Biomedical optics express, 2020, 11 (12), pp. 7204 - 7220
Source Title
Publisher
OPTICAL SOC AMER
ISSN
2156-7085
eISSN
2156-7085