Quantitative imaging of single-cell phenotypes in cancer cells cultured on hydrogel surfaces.
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Embargo End Date
ICR Authors
Authors
Bousgouni, V
Bakal, C
Bakal, C
Document Type
Journal Article
Date
2023-03-17
Date Accepted
2022-11-24
Abstract
Small interfering RNA (siRNA) screening approaches used with quantitative single-cell analysis can uncover the roles of genes in cell morphogenesis. Here, we present a high-throughput automated phenotypic screening technique to quantify a single cell shape in cancer cells cultured on top of soft 3D hydrogels. We describe reverse transfection of cells with siRNAs and seeding of these cells on top of collagen, followed by image analysis to quantify morphology of a single cell and population levels in low-elasticity matrices. For complete details on the use and execution of this protocol, please refer to Bousgouni et al. (2022).1.
Citation
STAR Protocols, 2022, 4 (1), pp. 101942 -
Source Title
STAR Protocols
Publisher
ELSEVIER
ISSN
2666-1667
eISSN
2666-1667
2666-1667
2666-1667
Collections
Research Team
Dynamical Cell Systems