Bioluminescence Resonance Energy Transfer 2 (BRET2)-Based RAS Biosensors to Characterize RAS Inhibitors.
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ICR Authors
Authors
Bery, N
Rabbitts, TH
Rabbitts, TH
Document Type
Journal Article
Date
2019-06-01
Date Accepted
Abstract
Protein-protein interactions (PPIs) are principle biological processes that control normal cell growth, differentiation, and homeostasis but are also crucial in diseases such as malignancy, neuropathy, and infection. Despite the importance of PPIs in biology, this target class has been very challenging to convert to therapeutics. In the last decade, much progress has been made in the inhibition of PPIs involved in diseases, but many remain difficult such as RAS-effector interactions in cancers. We describe here a protocol for using Bioluminescence Resonance Energy Transfer 2 (BRET2)-based RAS biosensors to detect and characterize RAS PPI inhibition by macromolecules and small molecules. This method could be extended to any other small GTPases or any other PPIs of interest. © 2019 by John Wiley & Sons, Inc.
Citation
Current protocols in cell biology, 2019, 83 (1), pp. e83 - ?
DOI
Source Title
Publisher
Wiley
ISSN
1934-2500
eISSN
1934-2616
Collections
Research Team
Chromosomal Translocations and Intracellular Antibody Therapeutics