Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis.
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Authors
Lambros, MB
Seed, G
Sumanasuriya, S
Gil, V
Crespo, M
Fontes, M
Chandler, R
Mehra, N
Fowler, G
Ebbs, B
Flohr, P
Miranda, S
Yuan, W
Mackay, A
Ferreira, A
Pereira, R
Bertan, C
Figueiredo, I
Riisnaes, R
Rodrigues, DN
Sharp, A
Goodall, J
Boysen, G
Carreira, S
Bianchini, D
Rescigno, P
Zafeiriou, Z
Hunt, J
Moloney, D
Hamilton, L
Neves, RP
Swennenhuis, J
Andree, K
Stoecklein, NH
Terstappen, LWMM
de Bono, JS
Seed, G
Sumanasuriya, S
Gil, V
Crespo, M
Fontes, M
Chandler, R
Mehra, N
Fowler, G
Ebbs, B
Flohr, P
Miranda, S
Yuan, W
Mackay, A
Ferreira, A
Pereira, R
Bertan, C
Figueiredo, I
Riisnaes, R
Rodrigues, DN
Sharp, A
Goodall, J
Boysen, G
Carreira, S
Bianchini, D
Rescigno, P
Zafeiriou, Z
Hunt, J
Moloney, D
Hamilton, L
Neves, RP
Swennenhuis, J
Andree, K
Stoecklein, NH
Terstappen, LWMM
de Bono, JS
Document Type
Journal Article
Date
2018-11-15
Date Accepted
2018-07-18
Abstract
Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency.Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity.Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses.Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution. Clin Cancer Res; 24(22); 5635-44. ©2018 AACR.
Citation
Clinical cancer research : an official journal of the American Association for Cancer Research, 2018, 24 (22), pp. 5635 - 5644
Source Title
Publisher
AMER ASSOC CANCER RESEARCH
ISSN
1078-0432
eISSN
1557-3265
Research Team
Cancer Biomarkers
Prostate Cancer Targeted Therapy Group
Translational Therapeutics
Glioma Team
Prostate Cancer Targeted Therapy Group
Translational Therapeutics
Glioma Team