Focused Ultrasound-Mediated Hyperthermia in Vitro: An Experimental Arrangement for Treating Cells under Tissue-Mimicking Conditions.
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Embargo End Date
ICR Authors
Authors
Brüningk, SC
Rivens, I
Mouratidis, P
Ter Haar, G
Rivens, I
Mouratidis, P
Ter Haar, G
Document Type
Journal Article
Date
2019-12-01
Date Accepted
2019-06-18
Abstract
An experimental arrangement that allows in vitro exposure of cells to focused ultrasound-mediated hyperthermia (43°C-55°C) in a tissue-mimicking phantom with biological, acoustic and thermal properties comparable to those of human soft tissue is described. Cells were embedded in a compressed collagen gel, which was sandwiched between 6-mm-thick slices of biocompatible, acoustically absorbing and thermally tissue mimicking poly(vinyl alcohol) cryo-gel. To illustrate the system's potential, cells were exposed using a 1.66-MHz focused ultrasound beam (spatial-peak temporal-average intensities (ISPTA) = 900-1400 W/cm2) that traced out a circular trajectory (5-8 mm in diameter). Real-time temperature monitoring allowed cells to be exposed reproducibly to a pre-determined thermal dose. An experimental planning tool that estimates the thermal dose distribution throughout the sample and allows spatial correlation with cell position has been developed. Treatment response was evaluated qualitatively using microscopy and cell viability testing. This experimental arrangement has significant potential for future, biologically relevant, in vitro focused ultrasound-mediated hyperthermia studies.
Citation
Ultrasound in medicine & biology, 2019, 45 (12), pp. 3290 - 3297
Source Title
Publisher
ELSEVIER SCIENCE INC
ISSN
0301-5629
eISSN
1879-291X
Collections
Research Team
Radiotherapy Physics Modelling
Therapeutic Ultrasound
Therapeutic Ultrasound