Browsing by author "Roumeliotis, Theodoros"
Now showing items 1-20 of 31
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A molecular quantitative trait locus map for osteoarthritis.
Steinberg, J; Southam, L; Roumeliotis, TI; Clark, MJ; Jayasuriya, RL; et al. (NATURE PORTFOLIO, 2021-02-26)Osteoarthritis causes pain and functional disability for over 500 million people worldwide. To develop disease-stratifying tools and modifying therapies, we need a better understanding of the molecular basis of the disease ... -
An E2-ubiquitin thioester-driven approach to identify substrates modified with ubiquitin and ubiquitin-like molecules.
Bakos, G; Yu, L; Gak, IA; Roumeliotis, TI; Liakopoulos, D; et al. (NATURE PUBLISHING GROUP, 2018-11-14)Covalent modifications of proteins with ubiquitin and ubiquitin-like molecules are instrumental to many biological processes. However, identifying the E3 ligase responsible for these modifications remains a major bottleneck ... -
Aneuploidy tolerance caused by BRG1 loss allows chromosome gains and recovery of fitness.
Schiavoni, F; Zuazua-Villar, P; Roumeliotis, TI; Benstead-Hume, G; Pardo, M; et al. (NATURE PORTFOLIO, 2022-04-01)Aneuploidy results in decreased cellular fitness in many species and model systems. However, aneuploidy is commonly found in cancer cells and often correlates with aggressive growth, suggesting that the impact of aneuploidy ... -
Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination.
Liess, AKL; Kucerova, A; Schweimer, K; Yu, L; Roumeliotis, TI; et al. (CELL PRESS, 2019-08-06)Ubiquitin-conjugating enzymes (E2s) govern key aspects of ubiquitin signaling. Emerging evidence suggests that the activities of E2s are modulated by posttranslational modifications; the structural underpinnings, however, ... -
Cancer-associated FBXW7 loss is synthetic lethal with pharmacological targeting of CDC7.
Baxter, JS; Brough, R; Krastev, DB; Song, F; Sridhar, S; et al. (WILEY, 2023-10-22)The F-box and WD repeat domain containing 7 (FBXW7) tumour suppressor gene encodes a substrate-recognition subunit of Skp, cullin, F-box (SCF)-containing complexes. The tumour-suppressive role of FBXW7 is ascribed to its ... -
Characterization of proteome-size scaling by integrative omics reveals mechanisms of proliferation control in cancer.
Jones, I; Dent, L; Higo, T; Roumeliotis, T; Arias Garcia, M; et al. (AMER ASSOC ADVANCEMENT SCIENCE, 2023-01-25)Almost all living cells maintain size uniformity through successive divisions. Proteins that over and underscale with size can act as rheostats, which regulate cell cycle progression. Using a multiomic strategy, we leveraged ... -
Citrobacter rodentium induces rapid and unique metabolic and inflammatory responses in mice suffering from severe disease.
Carson, D; Barry, R; Hopkins, EGD; Roumeliotis, TI; García-Weber, D; et al. (WILEY-HINDAWI, 2020-01-01)The mouse pathogen Citrobacter rodentium is used to model infections with enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC). Pathogenesis is commonly modelled in mice developing mild disease (e.g., ... -
Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo.
Berger, CN; Crepin, VF; Roumeliotis, TI; Wright, JC; Carson, D; et al. (CELL PRESS, 2017-11-07)The intestinal epithelial cells (IECs) that line the gut form a robust line of defense against ingested pathogens. We investigated the impact of infection with the enteric pathogen Citrobacter rodentium on mouse IEC ... -
Clustering of Tir during enteropathogenic E. coli infection triggers calcium influx-dependent pyroptosis in intestinal epithelial cells.
Zhong, Q; Roumeliotis, TI; Kozik, Z; Cepeda-Molero, M; Fernández, LÁ; et al. (PUBLIC LIBRARY SCIENCE, 2020-12-30)Clustering of the enteropathogenic Escherichia coli (EPEC) type III secretion system (T3SS) effector translocated intimin receptor (Tir) by intimin leads to actin polymerisation and pyroptotic cell death in macrophages. ... -
Evaluation of a Dual Isolation Width Acquisition Method for Isobaric Labeling Ratio Decompression.
Roumeliotis, TI; Weisser, H; Choudhary, JS (AMER CHEMICAL SOC, 2019-03-01)Isobaric labeling is a highly precise approach for protein quantification. However, due to the isolation interference problem, isobaric tagging suffers from ratio underestimation at the MS2 level. The use of narrow isolation ... -
Exploiting induced pluripotent stem cell-derived macrophages to unravel host factors influencing Chlamydia trachomatis pathogenesis.
Yeung, ATY; Hale, C; Lee, AH; Gill, EE; Bushell, W; et al. (NATURE PUBLISHING GROUP, 2017-04-25)Chlamydia trachomatis remains a leading cause of bacterial sexually transmitted infections and preventable blindness worldwide. There are, however, limited in vitro models to study the role of host genetics in the response ... -
Genomic Determinants of Protein Abundance Variation in Colorectal Cancer Cells.
Roumeliotis, TI; Williams, SP; Gonçalves, E; Alsinet, C; Del Castillo Velasco-Herrera, M; et al. (CELL PRESS, 2017-08-29)Assessing the impact of genomic alterations on protein networks is fundamental in identifying the mechanisms that shape cancer heterogeneity. We have used isobaric labeling to characterize the proteomic landscapes of 50 ... -
HSP90-CDC37-PP5 forms a structural platform for kinase dephosphorylation.
Oberoi, J; Guiu, XA; Outwin, EA; Schellenberger, P; Roumeliotis, TI; et al. (NATURE PORTFOLIO, 2022-11-29)Activation of client protein kinases by the HSP90 molecular chaperone system is affected by phosphorylation at multiple sites on HSP90, the kinase-specific co-chaperone CDC37, and the kinase client itself. Removal of ... -
A HUWE1 defect causes PARP inhibitor resistance by modulating the BRCA1-∆11q splice variant.
Pettitt, SJ; Shao, N; Zatreanu, D; Frankum, J; Bajrami, I; et al. (SPRINGERNATURE, 2023-09-01)Although PARP inhibitors (PARPi) now form part of the standard-of-care for the treatment of homologous recombination defective cancers, de novo and acquired resistance limits their overall effectiveness. Previously, ... -
Integrative epigenomics, transcriptomics and proteomics of patient chondrocytes reveal genes and pathways involved in osteoarthritis.
Steinberg, J; Ritchie, GRS; Roumeliotis, TI; Jayasuriya, RL; Clark, MJ; et al. (NATURE PORTFOLIO, 2017-08-21)Osteoarthritis (OA) is a common disease characterized by cartilage degeneration and joint remodeling. The underlying molecular changes underpinning disease progression are incompletely understood. We investigated genes and ... -
Interferon-driven alterations of the host's amino acid metabolism in the pathogenesis of typhoid fever.
Blohmke, CJ; Darton, TC; Jones, C; Suarez, NM; Waddington, CS; et al. (ROCKEFELLER UNIV PRESS, 2016-05-30)Enteric fever, caused by Salmonella enterica serovar Typhi, is an important public health problem in resource-limited settings and, despite decades of research, human responses to the infection are poorly understood. In ... -
Intestinal Epithelial Cells and the Microbiome Undergo Swift Reprogramming at the Inception of Colonic Citrobacter rodentium Infection.
Hopkins, EGD; Roumeliotis, TI; Mullineaux-Sanders, C; Choudhary, JS; Frankel, G (AMER SOC MICROBIOLOGY, 2019-04-02)We used the mouse attaching and effacing (A/E) pathogen Citrobacter rodentium, which models the human A/E pathogens enteropathogenic Escherichia coli and enterohemorrhagic E. coli (EPEC and EHEC), to temporally resolve ... -
Mechanism of assembly, activation and lysine selection by the SIN3B histone deacetylase complex.
Wan, MSM; Muhammad, R; Koliopoulos, MG; Roumeliotis, TI; Choudhary, JS; et al. (NATURE PORTFOLIO, 2023-05-03)Lysine acetylation in histone tails is a key post-translational modification that controls transcription activation. Histone deacetylase complexes remove histone acetylation, thereby repressing transcription and regulating ... -
Overexpression of Claspin and Timeless protects cancer cells from replication stress in a checkpoint-independent manner.
Bianco, JN; Bergoglio, V; Lin, Y-L; Pillaire, M-J; Schmitz, A-L; et al. (NATURE PUBLISHING GROUP, 2019-02-22)Oncogene-induced replication stress (RS) promotes cancer development but also impedes tumor growth by activating anti-cancer barriers. To determine how cancer cells adapt to RS, we have monitored the expression of different ... -
PBAF loss leads to DNA damage-induced inflammatory signaling through defective G2/M checkpoint maintenance.
Feng, H; Lane, KA; Roumeliotis, TI; Jeggo, PA; Somaiah, N; et al. (COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT, 2022-07-28)The PBRM1 subunit of the PBAF (SWI/SNF) chromatin remodeling complex is mutated in ∼40% of clear cell renal cancers. PBRM1 loss has been implicated in responses to immunotherapy in renal cancer, but the mechanism is unclear. ...