Browsing by author "Morris, Edward"
Now showing items 1-20 of 22
-
A closed conformation of the Caenorhabditis elegans separase-securin complex.
Bachmann, G; Richards, MW; Winter, A; Beuron, F; Morris, E; et al. (ROYAL SOC, 2016-04-13)The protease separase plays a key role in sister chromatid disjunction and centriole disengagement. To maintain genomic stability, separase activity is strictly regulated by binding of an inhibitory protein, securin. Despite ... -
Cryo-EM structures of the XPF-ERCC1 endonuclease reveal how DNA-junction engagement disrupts an auto-inhibited conformation.
Jones, M; Beuron, F; Borg, A; Nans, A; Earl, CP; et al. (NATURE PUBLISHING GROUP, 2020-02-28)The structure-specific endonuclease XPF-ERCC1 participates in multiple DNA damage repair pathways including nucleotide excision repair (NER) and inter-strand crosslink repair (ICLR). How XPF-ERCC1 is catalytically activated ... -
High-resolution cryo-EM proteasome structures in drug development.
Morris, EP; da Fonseca, PCA (INT UNION CRYSTALLOGRAPHY, 2017-06-01)With the recent advances in biological structural electron microscopy (EM), protein structures can now be obtained by cryo-EM and single-particle analysis at resolutions that used to be achievable only by crystallographic ... -
Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA.
Kong, M; Cutts, EE; Pan, D; Beuron, F; Kaliyappan, T; et al. (CELL PRESS, 2020-07-02)Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin ... -
Myosin and Actin Filaments in Muscle: Structures and Interactions.
Squire, JM; Paul, DM; Morris, EP (SPRINGER INTERNATIONAL PUBLISHING AG, 2017-01-01)In the last decade, improvements in electron microscopy and image processing have permitted significantly higher resolutions to be achieved (sometimes <1 nm) when studying isolated actin and myosin filaments. In the case ... -
Nanostructures from Synthetic Genetic Polymers.
Taylor, AI; Beuron, F; Peak-Chew, S-Y; Morris, EP; Herdewijn, P; et al. (WILEY-V C H VERLAG GMBH, 2016-06-16)Nanoscale objects of increasing complexity can be constructed from DNA or RNA. However, the scope of potential applications could be enhanced by expanding beyond the moderate chemical diversity of natural nucleic acids. ... -
Nse5/6 is a negative regulator of the ATPase activity of the Smc5/6 complex.
Hallett, ST; Schellenberger, P; Zhou, L; Beuron, F; Morris, E; et al. (OXFORD UNIV PRESS, 2021-05-07)The multi-component Smc5/6 complex plays a critical role in the resolution of recombination intermediates formed during mitosis and meiosis, and in the cellular response to replication stress. Using recombinant proteins, ... -
Origin licensing requires ATP binding and hydrolysis by the MCM replicative helicase.
Coster, G; Frigola, J; Beuron, F; Morris, EP; Diffley, JFX (CELL PRESS, 2014-09-04)Loading of the six related Minichromosome Maintenance (MCM) proteins as head-to-head double hexamers during DNA replication origin licensing is crucial for ensuring once-per-cell-cycle DNA replication in eukaryotic cells. ... -
Prereplicative complexes assembled in vitro support origin-dependent and independent DNA replication.
On, KF; Beuron, F; Frith, D; Snijders, AP; Morris, EP; et al. (WILEY-BLACKWELL, 2014-03-18)Eukaryotic DNA replication initiates from multiple replication origins. To ensure each origin fires just once per cell cycle, initiation is divided into two biochemically discrete steps: the Mcm2-7 helicase is first loaded ... -
Relaxed and active thin filament structures; a new structural basis for the regulatory mechanism.
Paul, DM; Squire, JM; Morris, EP (ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017-03-01)The structures of muscle thin filaments reconstituted using skeletal actin and cardiac troponin and tropomyosin have been determined with and without bound Ca2+ using electron microscopy and reference-free single particle ... -
RET recognition of GDNF-GFRα1 ligand by a composite binding site promotes membrane-proximal self-association.
Goodman, KM; Kjær, S; Beuron, F; Knowles, PP; Nawrotek, A; et al. (CELL PRESS, 2014-09-25)The RET receptor tyrosine kinase is essential to vertebrate development and implicated in multiple human diseases. RET binds a cell surface bipartite ligand comprising a GDNF family ligand and a GFRα coreceptor, resulting ... -
Structural Basis for a Reciprocal Regulation between SCF and CSN
Enchev, RI; Scott, DC; da Fonseca, PCA; Schreiber, A; Monda, JK; et al. -
Structural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome.
Faull, SV; Lau, AMC; Martens, C; Ahdash, Z; Hansen, K; et al. (NATURE RESEARCH, 2019-08-23)Cullin-Ring E3 Ligases (CRLs) regulate a multitude of cellular pathways through specific substrate receptors. The COP9 signalosome (CSN) deactivates CRLs by removing NEDD8 from activated Cullins. Here we present structures ... -
Structural basis of RNA polymerase III transcription initiation.
Abascal-Palacios, G; Ramsay, EP; Beuron, F; Morris, E; Vannini, A (NATURE PORTFOLIO, 2018-01-17)RNA polymerase (Pol) III transcribes essential non-coding RNAs, including the entire pool of transfer RNAs, the 5S ribosomal RNA and the U6 spliceosomal RNA, and is often deregulated in cancer cells. The initiation of gene ... -
Structural basis of tankyrase activation by polymerization.
Pillay, N; Mariotti, L; Zaleska, M; Inian, O; Jessop, M; et al. (NATURE PORTFOLIO, 2022-12-01)The poly-ADP-ribosyltransferase tankyrase (TNKS, TNKS2) controls a wide range of disease-relevant cellular processes, including WNT-β-catenin signalling, telomere length maintenance, Hippo signalling, DNA damage repair and ... -
Structure of human RNA polymerase III.
Ramsay, EP; Abascal-Palacios, G; Daiß, JL; King, H; Gouge, J; et al. (NATURE RESEARCH, 2020-12-17)In eukaryotes, RNA Polymerase (Pol) III is specialized for the transcription of tRNAs and other short, untranslated RNAs. Pol III is a determinant of cellular growth and lifespan across eukaryotes. Upregulation of Pol III ... -
Structures of APC/C(Cdh1) with substrates identify Cdh1 and Apc10 as the D-box co-receptor.
da Fonseca, PCA; Kong, EH; Zhang, Z; Schreiber, A; Williams, MA; et al. (NATURE PUBLISHING GROUP, 2011-02-10)The ubiquitylation of cell-cycle regulatory proteins by the large multimeric anaphase-promoting complex (APC/C) controls sister chromatid segregation and the exit from mitosis. Selection of APC/C targets is achieved through ... -
Tankyrase Requires SAM Domain-Dependent Polymerization to Support Wnt-β-Catenin Signaling.
Mariotti, L; Templeton, CM; Ranes, M; Paracuellos, P; Cronin, N; et al. (CELL PRESS, 2016-08-04)The poly(ADP-ribose) polymerase (PARP) Tankyrase (TNKS and TNKS2) is paramount to Wnt-β-catenin signaling and a promising therapeutic target in Wnt-dependent cancers. The pool of active β-catenin is normally limited by ... -
The Interacting Head Motif Structure Does Not Explain the X-Ray Diffraction Patterns in Relaxed Vertebrate (Bony Fish) Skeletal Muscle and Insect (Lethocerus) Flight Muscle.
Knupp, C; Morris, E; Squire, JM (MDPI, 2019-09-14)Unlike electron microscopy, which can achieve very high resolution but to date can only be used to study static structures, time-resolved X-ray diffraction from contracting muscles can, in principle, be used to follow the ... -
Three-dimensional structure of recombinant type 1 inositol 1,4,5-trisphosphate receptor.
Wolfram, F; Morris, E; Taylor, CW (PORTLAND PRESS LTD, 2010-05-27)IP3Rs (inositol 1,4,5-trisphosphate receptors) are the intracellular channels that mediate release of Ca2+ from the endoplasmic reticulum in response to the many stimuli that evoke Ins(1,4,5)P3 formation. We characterized ...