Now showing items 1-12 of 12

    • A closed conformation of the Caenorhabditis elegans separase-securin complex. 

      Bachmann, G; Richards, MW; Winter, A; Beuron, F; Morris, E; Bayliss, R (2016-04-13)
      The protease separase plays a key role in sister chromatid disjunction and centriole disengagement. To maintain genomic stability, separase activity is strictly regulated by binding of an inhibitory protein, securin. Despite ...
    • Cryo-EM structures of the XPF-ERCC1 endonuclease reveal how DNA-junction engagement disrupts an auto-inhibited conformation. 

      Jones, M; Beuron, F; Borg, A; Nans, A; Earl, CP; Briggs, DC; Snijders, AP; Bowles, M; Morris, EP; Linch, M; McDonald, NQ (2020-02-28)
      The structure-specific endonuclease XPF-ERCC1 participates in multiple DNA damage repair pathways including nucleotide excision repair (NER) and inter-strand crosslink repair (ICLR). How XPF-ERCC1 is catalytically activated ...
    • Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA. 

      Kong, M; Cutts, EE; Pan, D; Beuron, F; Kaliyappan, T; Xue, C; Morris, EP; Musacchio, A; Vannini, A; Greene, EC (2020-07)
      Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin ...
    • Nanostructures from Synthetic Genetic Polymers. 

      Taylor, AI; Beuron, F; Peak-Chew, S-Y; Morris, EP; Herdewijn, P; Holliger, P (2016-06)
      Nanoscale objects of increasing complexity can be constructed from DNA or RNA. However, the scope of potential applications could be enhanced by expanding beyond the moderate chemical diversity of natural nucleic acids. ...
    • Nse5/6 is a negative regulator of the ATPase activity of the Smc5/6 complex. 

      Hallett, ST; Schellenberger, P; Zhou, L; Beuron, F; Morris, E; Murray, JM; Oliver, AW
      The multi-component Smc5/6 complex plays a critical role in the resolution of recombination intermediates formed during mitosis and meiosis, and in the cellular response to replication stress. Using recombinant proteins, ...
    • Origin licensing requires ATP binding and hydrolysis by the MCM replicative helicase. 

      Coster, G; Frigola, J; Beuron, F; Morris, EP; Diffley, JFX (2014-09)
      Loading of the six related Minichromosome Maintenance (MCM) proteins as head-to-head double hexamers during DNA replication origin licensing is crucial for ensuring once-per-cell-cycle DNA replication in eukaryotic cells. ...
    • Prereplicative complexes assembled in vitro support origin-dependent and independent DNA replication. 

      On, KF; Beuron, F; Frith, D; Snijders, AP; Morris, EP; Diffley, JFX (2014-03)
      Eukaryotic DNA replication initiates from multiple replication origins. To ensure each origin fires just once per cell cycle, initiation is divided into two biochemically discrete steps: the Mcm2-7 helicase is first loaded ...
    • RET recognition of GDNF-GFRα1 ligand by a composite binding site promotes membrane-proximal self-association. 

      Goodman, KM; Kjær, S; Beuron, F; Knowles, PP; Nawrotek, A; Burns, EM; Purkiss, AG; George, R; Santoro, M; Morris, EP; McDonald, NQ (2014-09-18)
      The RET receptor tyrosine kinase is essential to vertebrate development and implicated in multiple human diseases. RET binds a cell surface bipartite ligand comprising a GDNF family ligand and a GFRα coreceptor, resulting ...
    • Structural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome. 

      Faull, SV; Lau, AMC; Martens, C; Ahdash, Z; Hansen, K; Yebenes, H; Schmidt, C; Beuron, F; Cronin, NB; Morris, EP; Politis, A (2019-08-23)
      Cullin-Ring E3 Ligases (CRLs) regulate a multitude of cellular pathways through specific substrate receptors. The COP9 signalosome (CSN) deactivates CRLs by removing NEDD8 from activated Cullins. Here we present structures ...
    • Structural basis of RNA polymerase III transcription initiation. 

      Abascal-Palacios, G; Ramsay, EP; Beuron, F; Morris, E; Vannini, A (2018-01)
      RNA polymerase (Pol) III transcribes essential non-coding RNAs, including the entire pool of transfer RNAs, the 5S ribosomal RNA and the U6 spliceosomal RNA, and is often deregulated in cancer cells. The initiation of gene ...
    • Structure of human RNA polymerase III. 

      Ramsay, EP; Abascal-Palacios, G; Daiß, JL; King, H; Gouge, J; Pilsl, M; Beuron, F; Morris, E; Gunkel, P; Engel, C; Vannini, A (2020-12-17)
      In eukaryotes, RNA Polymerase (Pol) III is specialized for the transcription of tRNAs and other short, untranslated RNAs. Pol III is a determinant of cellular growth and lifespan across eukaryotes. Upregulation of Pol III ...
    • Tankyrase Requires SAM Domain-Dependent Polymerization to Support Wnt-β-Catenin Signaling. 

      Mariotti, L; Templeton, CM; Ranes, M; Paracuellos, P; Cronin, N; Beuron, F; Morris, E; Guettler, S (2016-08)
      The poly(ADP-ribose) polymerase (PARP) Tankyrase (TNKS and TNKS2) is paramount to Wnt-β-catenin signaling and a promising therapeutic target in Wnt-dependent cancers. The pool of active β-catenin is normally limited by ...