Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes.
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Date
2021-11-30Author
Abascal-Palacios, G
Jochem, L
Pla-Prats, C
Beuron, F
Vannini, A
Type
Journal Article
Metadata
Show full item recordAbstract
Retrotransposons are endogenous elements that have the ability to mobilise their DNA between different locations in the host genome. The Ty3 retrotransposon integrates with an exquisite specificity in a narrow window upstream of RNA Polymerase (Pol) III-transcribed genes, representing a paradigm for harmless targeted integration. Here we present the cryo-EM reconstruction at 4.0 Å of an active Ty3 strand transfer complex bound to TFIIIB transcription factor and a tRNA gene. The structure unravels the molecular mechanisms underlying Ty3 targeting specificity at Pol III-transcribed genes and sheds light into the architecture of retrotransposon machinery during integration. Ty3 intasome contacts a region of TBP, a subunit of TFIIIB, which is blocked by NC2 transcription regulator in RNA Pol II-transcribed genes. A newly-identified chromodomain on Ty3 integrase interacts with TFIIIB and the tRNA gene, defining with extreme precision the integration site position.
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Subject
Saccharomyces cerevisiae
RNA-Directed DNA Polymerase
RNA Polymerase II
RNA Polymerase III
Transcription Factor TFIIIB
Saccharomyces cerevisiae Proteins
Retroelements
RNA, Transfer
Genes, Fungal
Research team
Vannini Group
Language
eng
Date accepted
2021-11-15
License start date
2021-11-30
Citation
Nature communications, 2021, 12 (1), pp. 6992 - ?
Publisher
NATURE PORTFOLIO