dc.contributor.author | Abascal-Palacios, G | |
dc.contributor.author | Jochem, L | |
dc.contributor.author | Pla-Prats, C | |
dc.contributor.author | Beuron, F | |
dc.contributor.author | Vannini, A | |
dc.date.accessioned | 2022-02-18T11:20:25Z | |
dc.date.available | 2022-02-18T11:20:25Z | |
dc.date.issued | 2021-11-30 | |
dc.identifier.citation | Nature communications, 2021, 12 (1), pp. 6992 - ? | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/5025 | |
dc.identifier.eissn | 2041-1723 | |
dc.identifier.eissn | 2041-1723 | |
dc.identifier.doi | 10.1038/s41467-021-27338-w | |
dc.identifier.doi | 10.1038/s41467-021-27338-w | |
dc.description.abstract | Retrotransposons are endogenous elements that have the ability to mobilise their DNA between different locations in the host genome. The Ty3 retrotransposon integrates with an exquisite specificity in a narrow window upstream of RNA Polymerase (Pol) III-transcribed genes, representing a paradigm for harmless targeted integration. Here we present the cryo-EM reconstruction at 4.0 Å of an active Ty3 strand transfer complex bound to TFIIIB transcription factor and a tRNA gene. The structure unravels the molecular mechanisms underlying Ty3 targeting specificity at Pol III-transcribed genes and sheds light into the architecture of retrotransposon machinery during integration. Ty3 intasome contacts a region of TBP, a subunit of TFIIIB, which is blocked by NC2 transcription regulator in RNA Pol II-transcribed genes. A newly-identified chromodomain on Ty3 integrase interacts with TFIIIB and the tRNA gene, defining with extreme precision the integration site position. | |
dc.format | Electronic | |
dc.format.extent | 6992 - ? | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | NATURE PORTFOLIO | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Saccharomyces cerevisiae | |
dc.subject | RNA-Directed DNA Polymerase | |
dc.subject | RNA Polymerase II | |
dc.subject | RNA Polymerase III | |
dc.subject | Transcription Factor TFIIIB | |
dc.subject | Saccharomyces cerevisiae Proteins | |
dc.subject | Retroelements | |
dc.subject | RNA, Transfer | |
dc.subject | Genes, Fungal | |
dc.title | Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2021-11-15 | |
rioxxterms.version | VoR | |
rioxxterms.versionofrecord | 10.1038/s41467-021-27338-w | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2021-11-30 | |
dc.relation.isPartOf | Nature communications | |
pubs.issue | 1 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Structural Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Structural Biology/Vannini Group | |
pubs.publication-status | Published | |
pubs.volume | 12 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Vannini Group | |
dc.contributor.icrauthor | Abascal Palacios, Guillermo | |
dc.contributor.icrauthor | Beuron, Fabienne | |
dc.contributor.icrauthor | Vannini, Alessandro | |