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dc.contributor.authorDergai, O
dc.contributor.authorCousin, P
dc.contributor.authorGouge, J
dc.contributor.authorSatia, K
dc.contributor.authorPraz, V
dc.contributor.authorKuhlman, T
dc.contributor.authorLhôte, P
dc.contributor.authorVannini, A
dc.contributor.authorHernandez, N
dc.date.accessioned2019-03-04T15:35:46Z
dc.date.issued2018-05-21
dc.identifier.citationGenes & development, 2018, 32 (9-10), pp. 711 - 722
dc.identifier.issn0890-9369
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3125
dc.identifier.eissn1549-5477en_US
dc.identifier.doi10.1101/gad.314245.118en_US
dc.description.abstractRNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and "proximal sequence element" (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the type 3 promoters only, of a TATA box, which determines Pol III specificity. To understand the mechanism by which the absence or presence of a TATA box results in specific Pol recruitment, we examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e., TATA-box-binding protein [TBP], TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific Pol recruitment. TFIIB and BRF2 could each, in a mutually exclusive fashion, be recruited to SNAPc. In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.
dc.formatPrint-Electronic
dc.format.extent711 - 722
dc.languageeng
dc.language.isoeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHumans
dc.subjectRNA Polymerase II
dc.subjectRNA Polymerase III
dc.subjectTATA-Box Binding Protein
dc.subjectTranscription Factor TFIIB
dc.subjectTranscription Factors
dc.subjectRNA, Small Nuclear
dc.subjectTATA Box
dc.subjectProtein Binding
dc.subjectProtein Transport
dc.subjectMutation
dc.subjectPromoter Regions, Genetic
dc.subjectHEK293 Cells
dc.subjectProtein Domains
dc.titleMechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters.
dc.typeJournal Article
dcterms.dateAccepted2018-04-17
rioxxterms.versionofrecord10.1101/gad.314245.118
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2018-05-21en_US
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfGenes & development
pubs.issue9-10
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology/Vannini Group
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology/Vannini Group
pubs.publication-statusPublished
pubs.volume32en_US
pubs.embargo.termsNot known
icr.researchteamVannini Groupen_US
dc.contributor.icrauthorVannini, Alessandroen


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