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dc.contributor.authorJunior, RP
dc.contributor.authorSonehara, NM
dc.contributor.authorJardim-Perassi, BV
dc.contributor.authorPal, A
dc.contributor.authorAsad, Y
dc.contributor.authorAlmeida Chuffa, LG
dc.contributor.authorChammas, R
dc.contributor.authorRaynaud, FI
dc.contributor.authorZuccari, DAPC
dc.date.accessioned2022-04-13T09:33:51Z
dc.date.available2022-04-13T09:33:51Z
dc.date.issued2022-01-19
dc.identifier.citationScientific reports, 2022, 12 (1), pp. 1008 - ?
dc.identifier.issn2045-2322
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/5084
dc.identifier.eissn2045-2322
dc.identifier.eissn2045-2322
dc.identifier.doi10.1038/s41598-022-04994-6
dc.identifier.doi10.1038/s41598-022-04994-6
dc.description.abstractHuman xenografts are extremely useful models to study the biology of human cancers and the effects of novel potential therapies. Deregulation of metabolism, including changes in amino acids (AAs), is a common characteristic of many human neoplasms. Plasma AAs undergo daily variations, driven by circadian endogenous and exogenous factors. We compared AAs concentration in triple negative breast cancer MDA-MB-231 cells and MCF10A non-tumorigenic immortalized breast epithelial cells. We also measured plasma AAs in mice bearing xenograft MDA-MB-231 and compared their levels with non-tumor-bearing control animals over 24 h. In vitro studies revealed that most of AAs were significantly different in MDA-MB-231 cells when compared with MCF10A. Plasma concentrations of 15 AAs were higher in cancer cells, two were lower and four were observed to shift across 24 h. In the in vivo setting, analysis showed that 12 out of 20 AAs varied significantly between tumor-bearing and non-tumor bearing mice. Noticeably, these metabolites peaked in the dark phase in non-tumor bearing mice, which corresponds to the active time of these animals. Conversely, in tumor-bearing mice, the peak time occurred during the light phase. In the early period of the light phase, these AAs were significantly higher in tumor-bearing animals, yet significantly lower in the middle of the light phase when compared with controls. This pilot study highlights the importance of well controlled experiments in studies involving plasma AAs in human breast cancer xenografts, in addition to emphasizing the need for more precise examination of exometabolomic changes using multiple time points.
dc.formatElectronic
dc.format.extent1008 - ?
dc.languageeng
dc.language.isoeng
dc.publisherNATURE PORTFOLIO
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCell Line
dc.subjectCell Line, Tumor
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectBreast Neoplasms
dc.subjectMammary Neoplasms, Experimental
dc.subjectAmino Acids
dc.subjectPilot Projects
dc.subjectNeoplasm Transplantation
dc.subjectCircadian Rhythm
dc.subjectFemale
dc.subjectTriple Negative Breast Neoplasms
dc.titlePresence of human breast cancer xenograft changes the diurnal profile of amino acids in mice.
dc.typeJournal Article
dcterms.dateAccepted2022-01-04
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1038/s41598-022-04994-6
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2022-01-19
dc.relation.isPartOfScientific reports
pubs.issue1
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Therapeutics
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Clinical Pharmacology & Trials (including Drug Metabolism & Pharmacokinetics Group)
pubs.publication-statusPublished
pubs.volume12
pubs.embargo.termsNot known
icr.researchteamClinical Pharmacology & Trials (including Drug Metabolism & Pharmacokinetics Group)
dc.contributor.icrauthorPal, Akos
dc.contributor.icrauthorRaynaud, Florence


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