dc.contributor.author | Coker, EA | |
dc.contributor.author | Stewart, A | |
dc.contributor.author | Ozer, B | |
dc.contributor.author | Minchom, A | |
dc.contributor.author | Pickard, L | |
dc.contributor.author | Ruddle, R | |
dc.contributor.author | Carreira, S | |
dc.contributor.author | Popat, S | |
dc.contributor.author | O'Brien, M | |
dc.contributor.author | Raynaud, F | |
dc.contributor.author | de Bono, J | |
dc.contributor.author | Al-Lazikani, B | |
dc.contributor.author | Banerji, U | |
dc.date.accessioned | 2022-05-17T12:01:28Z | |
dc.date.available | 2022-05-17T12:01:28Z | |
dc.date.issued | 2022-06-01 | |
dc.identifier.citation | Molecular cancer therapeutics, 2022 | |
dc.identifier.issn | 1535-7163 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/5130 | |
dc.identifier.eissn | 1538-8514 | |
dc.identifier.eissn | 1538-8514 | |
dc.identifier.doi | 10.1158/1535-7163.mct-21-0442 | |
dc.identifier.doi | 10.1158/1535-7163.mct-21-0442 | |
dc.description.abstract | We hypothesize that the study of acute protein perturbation in signal transduction by targeted anticancer drugs can predict drug sensitivity of these agents used as single agents and rational combination therapy. We assayed dynamic changes in 52 phosphoproteins caused by an acute exposure (1 hour) to clinically relevant concentrations of seven targeted anticancer drugs in 35 non-small cell lung cancer (NSCLC) cell lines and 16 samples of NSCLC cells isolated from pleural effusions. We studied drug sensitivities across 35 cell lines and synergy of combinations of all drugs in six cell lines (252 combinations). We developed orthogonal machine-learning approaches to predict drug response and rational combination therapy. Our methods predicted the most and least sensitive quartiles of drug sensitivity with an AUC of 0.79 and 0.78, respectively, whereas predictions based on mutations in three genes commonly known to predict response to the drug studied, for example, EGFR, PIK3CA, and KRAS, did not predict sensitivity (AUC of 0.5 across all quartiles). The machine-learning predictions of combinations that were compared with experimentally generated data showed a bias to the highest quartile of Bliss synergy scores (P = 0.0243). We confirmed feasibility of running such assays on 16 patient samples of freshly isolated NSCLC cells from pleural effusions. We have provided proof of concept for novel methods of using acute ex vivo exposure of cancer cells to targeted anticancer drugs to predict response as single agents or combinations. These approaches could complement current approaches using gene mutations/amplifications/rearrangements as biomarkers and demonstrate the utility of proteomics data to inform treatment selection in the clinic. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | AMER ASSOC CANCER RESEARCH | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.title | Individualized Prediction of Drug Response and Rational Combination Therapy in NSCLC Using Artificial Intelligence-Enabled Studies of Acute Phosphoproteomic Changes. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2022-03-11 | |
rioxxterms.version | AM | |
rioxxterms.versionofrecord | 10.1158/1535-7163.mct-21-0442 | |
rioxxterms.licenseref.startdate | 2022-04-03 | |
dc.relation.isPartOf | Molecular cancer therapeutics | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/ImmNet | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Cancer Biomarkers | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Clinical Pharmacology & Trials (including Drug Metabolism & Pharmacokinetics Group) | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Computational Biology and Chemogenomics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Clinical Studies | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Clinical Studies/Cancer Biomarkers | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Clinical Studies/Clinical Pharmacology – Adaptive Therapy | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Clinical Studies/Prostate Cancer Targeted Therapy Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Clinical Studies/The Adult Drug Development Unit at the ICR and the RM | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Clinical Studies/Thoracic Oncology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Clinical Studies/Thoracic Oncology/Thoracic Oncology (hon.) | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Clinical Studies/Treatment of thoracic tumours | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Clinical Studies/Treatment of thoracic tumours/Treatment of thoracic tumours (hon.) | |
pubs.organisational-group | /ICR/Primary Group/Royal Marsden Clinical Units | |
pubs.publication-status | Published | |
pubs.embargo.terms | Not known | |
icr.researchteam | Clinical Pharmacology & Trials (including Drug Metabolism & Pharmacokinetics Group) | |
icr.researchteam | Computational Biology and Chemogenomics | |
icr.researchteam | Cancer Biomarkers | |
icr.researchteam | Clinical Pharmacology – Adaptive Therapy | |
icr.researchteam | Prostate Cancer Targeted Therapy Group | |
icr.researchteam | The Adult Drug Development Unit at the ICR and the RM | |
icr.researchteam | Thoracic Oncology | |
icr.researchteam | Treatment of thoracic tumours | |
dc.contributor.icrauthor | Minchom, Anna | |
dc.contributor.icrauthor | Ruddle, Ruth | |
dc.contributor.icrauthor | Carreira, Suzanne | |
dc.contributor.icrauthor | Raynaud, Florence | |
dc.contributor.icrauthor | De Bono, Johann | |
dc.contributor.icrauthor | Al-Lazikani, Bissan | |
dc.contributor.icrauthor | Banerji, Udai | |